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AOAC (2006) Official Methods of Analysis. 18th Edition, Association of Official Analytical Chemists, Gaithersburgs, MD.

has been cited by the following article:

  • TITLE: Decrease in Lysine and Tryptophan Content in S2 Inbred Lines from a Quality Protein Maize (QPM) Variety in a Breeding Program

    AUTHORS: Kabwe Nkongolo, Kankolongo Mbuya

    KEYWORDS: Quality Protein Maize, Lysine, Tryptophan, Amino Acid Profile, Inbred, Maize Synthetic Population, MUDISHI 3, DR-Congo

    JOURNAL NAME: American Journal of Plant Sciences, Vol.6 No.1, January 15, 2015

    ABSTRACT: Several countries in Africa, Latin America along with China have incorporated QPM in their Agricultural development plan. A new quality protein maize variety (QPM) was developed by breeders and farmers using the participatory breeding approach in the DR-Congo. It is adapted to all the maize growing regions in the country. Inbred lines from this new variety were produced for further development of maize synthetic populations. The main objective of the present study is to determine the level of amino acid changes in early generations of inbred lines. The results of the study revealed a significant decrease of 33% and 38% of tryptophan in S1 and S2 inbred lines compared to the original parental MUDISHI 3 population, respectively. There was a decrease of 15% of lysine in S2 inbred lines compared to the parental MUDISHI 3. Actually, S2 inbred lines of MUDISHI 3 contain similar level of lysine compared to the genetically improved normal maize (Salongo 2) that is currently released. The development of composite lines is recommended over synthetic populations to maintain the high levels of lysine and tryptophan along with other desirable agronomic characteristics since they involve the intercrossing of open pollinated varieties.