L-ornithine hydrochloride ingestion increased carbohydrate oxidation but not lipid oxidation during submaximal endurance exercise following resistance exercise

Shinichi Demura, Takayoshi Yamada, Shunsuke Yamaji, Masanobu Uchiyama

Faculty of Medicine, Fukui University, Fukui, Japan.
General Education Center, Fukui National College of Technology, Fukui, Japan.
Graduate School of Natural Science & Technology, Kanazawa University, Kanazawa, Japan.
Research and Education Center for Comprehensive Science, Akita Prefectural University, Akita, Japan.

DOI: 10.4236/abb.2013.41012                

Abstract

This study aimed to examine the effect of L-ornithine hydrochloride ingestion on lipid oxidation during submaximal endurance exercise following resistance exercise. Ten healthy young male adults with no history of regular resistance exercise (age: 26.0 +/- 4.4) conducted resistance exercise after L-ornithine hydrochloride or placebo ingestion (0.1 g/kg). Subjects exercised for 60 min on an ergometer at 50% VO_2peak 120 min after resistance exercise. Plasma ornithine concentrations measured immediately, 120min and 180min after resistance exercise in the L-ornithine hydrochloride ingestion condition were significantly greater than those in the placebo condition. No significant difference was found in serum growth hor mone concentrations between both conditions (F = 4.4, p = 0.065). Serum free fatty acid concentrations were significantly greater immediately after submaximal ergometer bicycle exercise in both conditions than those before ingestion, immediately after resistance exercise and 120min after resistance exercise (F = 43.4, p < 0.001, 300% - 508%), but no significant difference was found between both conditions (F = 3.6, p = 0.090). A similar trend was observed in serum ketone bodies as well. Although total energy production during submaximal ergometer exercise did not significantly differ (t = 0.74, p = 0.238), a significant difference was found in energy production via carbohydrate and lipid oxidation; the former was greater in the Lornithine hydrochloride ingestion condition (t = 1.89, p = 0.046, d = 0.44, 106%), and the latter was greater in the placebo condition (t = 1.89, p = 0.046, d = 0.78%, 145%). From the above, L-ornithine hydrochloride ingestion may not affect lipid metabolism during submaximal endurance exercise following resistance exercise. It may be involved in energy production via carbohydrate oxidation with glucogenic amino acid.

Keywords

L-Ornithine Hydrochloride; Endurance Exercise; Lipid Oxidation

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Poirier, P. and Després, J.P. (2001) Exercise in weight management of obesity. Cardiology Clinics, 19, 459-470. doi:10.1016/S0733-8651(05)70229-0
[2]	Santa-Clara, H., Fernhall, B., Baptista, F., Mendes, M. and Sardinha, L.B. (2003) Effect of a one-year combined exercise training program on body composition in men with coronary artery disease. Metabolism, 52, 1413-1417. doi:10.1016/S0026-0495(03)00320-2
[3]	Izquierdo, M., Iba?ez, J., HAkkinen, K., Kraemer, W.J., Larrión, J.L. and Gorostiaga, E.M. (2004) Once weekly combined resistance and cardiovascular training in healthy older men. Medicine and Science in Sports and Exercise, 36, 435-443. doi:10.1249/01.MSS.0000117897.55226.9A
[4]	Lambers, S., Van Laethem, C., Van Acker, K. and Calders, P. (2008) Influence of combined exercise training on indices of obesity, diabetes and cardiovascular risk in type 2 diabetes patients. Clinical Rehabilitation, 22, 483-492. doi:10.1177/0269215508084582
[5]	Goto, K., Higashiyama, M., Ishii, N. and Takamatsu, K. (2005) Prior endurance exercise attenuates growth hormone response to subsequent resistance exercise. European Journal of Applied Physiology, 94, 333-338. doi:10.1007/s00421-004-1296-x
[6]	Kraemer, W.J., Marchitelli, L., Gordon, S.E., Harman, E., Dziados, J.E., Mello, R., Frykman, P., McCurry, D. and Fleck, S.J. (1990) Hormonal and growth factor responses to heavy resistance exercise protocols. Journal of Applied Physiology, 69, 1442-1150.
[7]	M?ller, N., J?rgensen, J.O., Alberti, K.G., Flyvbjerg, A. and Schmitz, O. (1990) Short-term effects of growth hormone on fuel oxidation and regional substrate metabolism in normal man. Journal of Clinical Endocrinology and Metabolism, 70, 1179-1186. doi:10.1210/jcem-70-4-1179
[8]	Quisth, V., Enoksson, S., Blaak, E., Hagstr?m-Toft, E., Arner, P. and Bolinder, J. (2005) Major differences in noradrenaline action on lipolysis and blood flow rates in skeletal muscle and adipose tissue in vivo. Diabetologia, 48, 946-953. doi:10.1007/s00125-005-1708-4
[9]	Stich, V., de Glisezinski, I., Berlan, M., Bulow, J., Galitzky, J., Harant, I., Suljkovicova, H., Lafontan, M., Rivière, D. and Crampes, F. (2000) Adipose tissue lipolysis is increased during a repeated bout of aerobic exercise. Journal of Applied Physiology, 88, 1277-1283.
[10]	Pritzlaff, C.J., Wideman, L., Blumer, J., Jensen, M., Abbott, R.D., Gaesser, G.A., Veldhuis, J.D. and Weltman, A. (2000) Catecholamine release, growth hormone secretion, and energy expenditure during exercise vs. recovery in men. Journal of Applied Physiology, 89, 937-946.
[11]	Lambert, M.I., Hefer, J.A., Millar, R.P. and Macfarlane, P.W. (1993) Failure of commercial oral amino acid supplements to increase serum growth hormone concentrations in male body-builders. International Journal of Sport Nutrition, 3, 298-305.
[12]	Fry, A.C., Kraemer, W.J., Stone, M.H., Warren, B.J., Kearney, J.T., Maresh, C.M., Weseman, C.A. and Fleck, S.J. (1993) Endocrine and performance responses to high volume training and amino acid supplementation in elite junior weightlifters. International Journal of Sport Nutrition, 3, 306-322.
[13]	Cynober, L. (2007) Pharmacokinetics of arginine and related amino acids. Journal of Nutrition, 137, 1646S-1649S.
[14]	Cynober, L., Coudray-Lucas, C., de Bandt, J.P., Guéchot, J., Aussel, C., Salvucci, M. and Giboudeau, J. (1990) Action of ornithine alpha-ketoglutarate, ornithine hydrochloride, and calcium alpha-ketoglutarate on plasma amino acid and hormonal patterns in healthy subjects. Journal of the American College of Nutrition, 9, 2-12.
[15]	Bucci, L., Hickson, J.F., Pivarnik, J.M., Wolinsky, I., Mc Mahon, J.C. and Turner, S.D. (1990) Ornithine ingestion and growth hormone release in bodybuilders. Nutrition Research, 10, 239-245.
[16]	Demura, S., Yamada, T., Yamaji, S., Komatsu, M. and Morishita, K. (2010a) The effect of L-ornithine hydrochloride ingestion on human growth hormone secretion after strength training. Advances in Bioscience and Biotechnology, 1, 7-11. doi:10.4236/abb.2010.11002
[17]	Binzen, C., Swan, P. and Manore, M. (2001) Postexercise oxygen consumption and substrate use after resistance exercise in women. Medicine and Science in Sports Exercise, 33, 932-938. doi:10.1097/00005768-200106000-00012
[18]	Melby, C., Scholl, C., Edwards, G. and Bullough, R. (1993) Effect of acute resistance exercise on postexercise energy expenditure and resting metabolic rate. Journal of Applied Physiology, 75, 1847-1853.
[19]	Ormsbee, M., Thyfault, J., Johnson, E., Kraus, R., Choi, M. and Hickner, R. (2007) Fat metabolism and acute resistance exercise in trained men. Journal of Applied Physiology, 102, 1767-1772. doi:10.1152/japplphysiol.00704.2006
[20]	Melanson, E., Sharp, T., Seagle, H., Donahoo, W., Grunwald, G., Peters, J., Hamilton, J. and Hill, J. (2002) Resistance and aerobic exercise have similar effects on 24-h nutrient oxidation. Medicine and Science in Sports and Exercise, 34, 1793-1800. doi:10.1097/00005768-200211000-00016
[21]	Gravh?lt, C.H., Schmitz, O., Simonsen, L., Bülow, J., Christiansen, J.S. and M?ller, N. (1990) Effects of a physiological GH pulse on interstitial glycerol in abdominal and femoral adipose tissue. American Journal of Physiology, 277, E848-E854.
[22]	Thyfault, J.P., Carper, M.J., Richmond, S.R., Hulver, M.W. and Potteiger, J.A. (2004) Effects of liquid carbohydrate ingestion on markers of anabolism following high-intensity resistance exercise. Journal of Strength and Conditioning Research, 18, 174-179.
[23]	Kraemer, W.J., Noble, B.J., Clark, M.J. and Culver, B.W. (1987) Physiologic responses to heavy-resistance exercise with very short rest periods. International Journal of Sports Medicine, 8, 247-252. doi:10.1055/s-2008-1025663
[24]	Manetta, J., Brun, J.F., Perez-Martin, A., Callis, A., Pre faut, C. and Mercier, J. (2002) Fuel oxidation during exercise in middle-aged men: Role of training and glucose disposal. Medicine and Science in Sports and Exercise, 34, 423-429. doi:10.1097/00005768-200203000-00007
[25]	Beidleman, B.A., Rock, P.B., Muza, S.R., Fulco, C.S., Gibson, L.L., Kamimori, G.H. and Cymerman, A. (2002) Substrate oxidation is altered in women during exercise upon acute altitude exposure. Medicine and Science in Sports Exercise, 34, 430-437. doi:10.1097/00005768-200203000-00008
[26]	Marion-Latard, F., De Glisezinski, I., Crampes, F., Berlan, M., Galitzky, J., Suljkovicova, H., Riviere, D. and Stich, V. (2001) A single bout of exercise induces beta-adrenergic desensitization in human adipose tissue. American Journal of Physiology—Regulatory, Integrative and Comparative Physiology, 280, R166-R173.
[27]	Demura, S., Yamada, T., Yamaji, S., Komatsu, M. and Morishita, K. (2010b) The effect of L-ornithine hydrochloride ingestion on performance during incremental exhaustive ergometer bicycle exercise and ammonia metabolism during and after exercise. European Journal of Clinical Nutrition, 64, 1166-1171. doi:10.1038/ejcn.2010.149