In this study, a derivative spectrophotometric method and one HPLC method were developed and validated for analysis of anti-diabetic drugs, repaglinide (RPG) and metformine hydrochloride (MTF) in tablets. The spectrophotometric methods were based on zero-crossing first-derivative and fourth-derivative spectrophotometric method for simultaneous analysis of RPG (308 nm) and MTF (267 nm), respectively. Linear relationship between the absorbance at λ max and the drug concentration was found to be in the ranges of 5.0 - 50.0 μg ·mL -1 for both RPG and MTF. The quantification limits for RPG and MTF were found to be 0.568 and 1.156 μg ·mL -1, respectively. The detection limits were 0.170 and 0.347 μg ·mL -1 for RPG and MTF, respectively. The second method is a rapid stability-indicating isocratic HPLC method developed for the determination of RPG and MTF. A linear response was observed within the concentration range of 5.0 - 50.0 μg ·mL -1 for both RPG and MTF. The quantification limits for RPG and MTF were found to be 1.821 and 1.653 μg ·mL -1, respectively. The detection limits were 0.601 and 0.545 μg ·mL -1 for RPG and MTF, respectively. The proposed methods were successfully applied to the tablet analysis with good accuracy and precision.
Oral hypoglycaemia agents consist of two major classes such as biguanides and sulfonylureas. Metformin and phenformin are the most commonly used drugs in biguanide class that have been established in the therapy of non-insulin-dependent diabetes mellitus for decades. However, after the introduction of sulfonylureas, biguanides were nearly eliminated from the market, largely because of the risk of severe lactic acidosis [
Glinides, the new class of insulin secretagogues, are named as such because of organic structural similarities to meglitinides. Repaglinide (
Several analytical methods have been reported for the determination of metformin such as liquid chromatography-tandem mass spectrometry [
developed and validated for simultaneous determination of RPG and metformin hydrochloride MTF in original fixed dose combination tablet preparations using a zero-crossing first-derivative for RPG and fourth-derivative for MTF accurately. The derivative UV-spectrophotometric method is very simple and requires no reagent, pH-adjustment and extraction as compared to chromatographic technique, and can be used for routine analysis of RPG and MTF, simultaneously in fixed dose combination tablets in quality control laboratories.
In the second method, a simple, accurate, reproducible and rapid HPLC method with good resolution was developed and validated for determination of RPG and MTF in original tablets unaffected by interferences from the excipients without using an internal standard. The method proved is selective and useful for the investigation of the stability of RPG and MTF.
RPG and MTF were kindly supplied by Zentiva Ilac (Istanbul, Turkey) and Deva Ilac (Istanbul, Turkey), respectively. Their combined pharmaceutical preparation Prandimet film tablet®, containing 1 mg of RPG and 500 mg MTF per tablets was obtained from local pharmacy. All chemicals and reagents were of analytical-reagent grade.
For derivative spectrophotometric method, portions (10 mg each) of standard RPG and MTF were weighed and transferred to 2 separate 10 mL volumetric flasks and dissolved in methanol and further diluted with the same solvent to obtain standard solutions of RPG and MTF having final concentrations of 200 µg・mL−1 each of them.
For HPLC method, stock solutions of RPG and MTF were prepared in methanol to give a concentration of 1 mg・mL−1 of RPG and MTF and then diluted further with the mobile phase (acetonitrile-o-phosphoric acid (40:60), v/v) to obtain standard solutions of 200 µg・mL−1 each of them.
Spectrophotometric measurements were carried out using a Shimadzu UV-160 A spectrophotometer with 1-cm glass cells.
The HPLC analyses were performed on a Shimadzu LC-20A (Kyoto, Japan) which consisted of an LC-20AT solvent delivery system equipped with a Rheodyne injection valve, DGU-20A5 vacuum degasser, CTO-10ASVP column oven and SPDM20A photodiode array detector (PDA) at 225 nm. The data was collected and analyzed via the automation system software. Separations were performed at room temperature on a Luna C18 column (4.6 mm i.d. × 250 mm, 5 μm particle; Phenomenex, TX, USA), with a guard column (4 mm × 3 mm i.d.; Phenomenex) packed with the same material. The mobile phase consists of acetonitrile-o-phosphoric acid pH adjusted to 3 with 1 N NaOH (40:60), v/v at a flow rate of 1.0 mL・min−1. The mobile phase was degassed by an ultrasonic bath and filtered by a Millipore vacuum filter system equipped with a 0.45 mm HV filter.
Two diluted standards were prepared from the stock solutions of RPG and MTF, series A and B, respectively, as follows: Different aliquots of drug solution (0.125 - 1.25 mL) were transferred to 5 mL volumetric flask to provide final concentration range 5.0 - 50.0 µg・mL−1 and the volume was diluted to volume with methanol.
The absorbance of the standard solutions of RPG and MTF were measured at wavelengths of 308 nm and 267 nm for repaglinide and metformin, respectively.
Two diluted standards were prepared from the stock solutions of RPG (series A) and MTF (series B), as follows:
Series A: Different aliquots of RPG solution (0.125 - 1.25 mL) were transferred to 5 mL volumetric flask to provide final concentration range 5.0 - 50.0 µg・mL−1 and the volume was diluted to volume with mobile phase (acetonitrile-o- phosphoric acid (40:60), v/v).
Series B: Different aliquots of MTF solution (0.125 - 1.25 mL) were transferred to 5 mL volumetric flask to provide final concentration range 5.0 - 50.0 µg・mL−1 and the volume was diluted to volume with mobile phase (acetonitrile-o- phosphoric acid (40:60), v/v).
The absorbance of the standard solutions of RPG and MTF were measured at wavelength of 225 nm.
For derivative spectrophotometric method, ten tablets were weighed and finely powdered. Powder equivalent to 1.0 mg RPG and 500 mg MTF was accurately weighed and transferred to a 50 mL volumetric flask. 25 mL methanol was transferred to the volumetric flask and then and then extraction was performed mechanically for 20 min and sonicated for 20 more minutes. The volume was brought to 50 mL with same solvent and the content was centrifuged for 10 min. The stock solution containing 20 µg・mL−1 RPG and 10 mg・mL−1 MTF was used for the determination of RPG. From this solution, 1.0 mL was transferred to a 500 mL volumetric flask. The volume was diluted with methanol to the mark to give a solution containing 0.04 µg・mL−1 RPG and 20 µg・mL−1 MTF (Solution 1), which was used for the determination of MTF. RPG and MTF are present in very different quantities in the formulation which requires their measurement in different dilution levels to avoid any need for addition of RPG as standart to the sample solution; firstly RPG is measured at lower dilution then MTF at higher dilution.
For HPLC method, powder equivalent to 1.0 mg RPG and 500 mg MTF was accurately weighed and transferred to a 50 mL volumetric flask. 25 mL methanol was transferred to the volumetric flask and then extraction was performed mechanically for 20 min and sonicated for 20 more minutes. A 1 mL aliquot of the supernatant was diluted to 50 mL with the mobile phase. 20 µL of its aliquot was injected and chromatographed (n = 5) for the determination of RPG. From the solution above, 1 mL was transferred to a 500 mL volumetric flask. The volume was diluted with mobil phase to the mark to give a solution containing 0.04 µg・mL−1 RPG and 20 µg・mL−1 MTF. A 20 µl of its aliquot was injected and chromatographed (n = 5) for the determination of MTF.
RPG and MTF are oral hypoglycemic agents. A fixed dose combination of 500 mg of MTF and 1 mg of RPG is available commercially and indicated for the treatment of type 2 diabetes mellitus. For the simultaneous analysis of these drugs, a zero crossing first-derivative and fourth-derivative spectrophotometric method was developed in fixed dose combination tablet preparations. The absorption spectra of the two compounds, RPG and MTF were shown in
spectrophotometry based on a mathematical transformation of the zero-order curve into the derivative spectra can overcome that problem [
In order to separate RPG and MTF formed under given conditions, aqueous buffer-acetonitrile mixtures were used as the mobile phase. Satisfactory resolution
was obtained using the mobile phase system of acetonitrile-o-phosphoric acid (pH:3) (40:60, v/v) at a flow rate of 1.0 mL・min−1 using C18 column and UV detector was set at 225 nm. In
Linearity range
In the derivative spectrophotometric method, the absorbances of the standard solutions were measured at wavelengths of 308 nm and 267 nm for RPG and MTF, respectively. The calibration curves were constructed by plotting the D1 values against RPG and D4 values against MTF. The concentration ranges were found to be 5.0 - 50.0 µg・mL−1 for both RPG and MTF. In the HPLC method, the calibration curve was prepared by plotting the peak area of RPG and MTF against drug concentrations and they were linear within the range of 5.0 - 50.0 µg・mL−1. Peak areas and concentrations were subjected to least square linear regression analysis for the calculation of the calibration equation and correlation coefficients (
LOD and LOQ
The limits of detection (LOD) and limits of quantitation (LOQ) were determined using the formula: LOD or LOQ = kSDa/b, where k = 3 for LOD and 10 for LOQ, SDa is the standard deviation of the intercept, and b is the slope.
In the spectrophotometric methods, the LOD values were found to be 0.170 µg・mL−1 for RPG and 0.347 µg・mL−1 for MTF. The LOQ values were found to be 0.568 µg・mL−1 for RPG and 1.156 µg・mL−1 for MTF (n = 5). In the HPLC method, the LOD values were found to be 0.601 µg・mL−1 for RPG and 0.545 µg・mL−1 for MTF. The LOQ values were found to be 1.821 µg・mL−1 for RPG and 1.653 µg・mL−1 for MTF (n = 5) (
Precision
The inter- and intra-day precision studies were carried out by analysis of drugs for the same day and seven consecutive days (each n = 5). The RSD values for intra-day precision were 0.61% - 0.92% and interday precision was 1.18% - 1.91% for all developed methods indicating good precision. The obtained results are summarized in
Recovery
For recovery studies, the standard addition technique was applied. Certain amounts of pure sample solution was added to different concentrations of the standard drug mix solution and assayed. The percent recovery was was calculated from:
Recovery % = [ ( C t − C u ) / C a ] × 1 00
where C t is the total concentration the analyte found; C u is the concentration of the analyte present in the formulation; and C a is the concentration of the pure analyte added to the formulation. The results of recovery analysis are given in
Stability
The stability was tested at some storage conditions (room temperature in the dark for 48 h; autosampler conditions for 24 h and 4˚C for 1 week). As a result of stability studies the samples were stable when kept at room temperature in the dark for 48 h, in autosampler conditiıons 24 h and refrigerated at 4˚C for 1 week.