TITLE:
In Vitro and in Vivo Anti-Inflammatory Effect of a Biotechnologically Modified Borage Seed Extract: Evidence for Lipid Pro-Resolving Mediators’ Implication in the Enhancement of Psoriatic and Atopic Dermatitis Lesions
AUTHORS:
Gérald Chene, Vincent Baillif, Emeline Van Goethem, Jean-Eric Branka, Toni Ionescu, Géraldine Robert, Luc Lefeuvre
KEYWORDS:
Human Skin, Biotechnologically Modified Borage Extract, Interleukins, Inflammation, Psoriasis, Atopic Dermatitis, Resolvins, Maresins, Lipoxins
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.5 No.2,
June
29,
2015
ABSTRACT: Aim: Resolvins, maresins and lipoxins are lipid mediators issued from essential
polyunsaturated fatty acids which are the first anti-inflammatory and pro-resolving
signals identified during the resolution phase of inflammation. As borage oil and/or
borage seed extracts have shown beneficial action in treatment of atopic dermatitis
or eczema in human and canine, we have modified a borage oil component by using
biotechnology in order to get a compound structurally related to a polyunsaturated
fatty acid, and we have studied its ability to reduce inflammation mediators production
through the generation of resolvins, maresins and/or lipoxins. Additionally, we
have demonstrated the potent anti-inflammatory effect of this new compound which
consists in borage seed oil aminopropanediol amides, through an in vivo study concerning subjects suffering
from psoriasis or atopic dermatitis. Study Design/Methods: For the in vitro study, inflammation was induced
in co-cultures of human dendritic cells and normal keratinocytes by the addition
of PMA and the calcium ionophore A23187. Ability of our borage seed oil aminopropanediol
amides to increase resolvin D2, maresin 1 and lipoxins A4 and B4 synthesis was then
measured. Pro-inflammatory cytokines (IL-1β,
IL-6, IL-8) and PGE2 productions were also quantified. For the in vivo study, 36 subjects suffering from
psoriasis or atopic dermatitis have used twice a day during 30 days, a formulation
containing borage seed oil aminopropanediol amides. Before the beginning of the
study and after 30 days’ treatment, the severity of psoriasis and of atopic dermatitis
was evaluated by using the PGA and the SCORAD scoring scales, respectively. Results:
Borage seed oil aminopropanediol amides were able to significantly increase the
resolvin D2, maresin 1 and lipoxins A4 and B4 synthesis. Concomitantly, they were
also able to significantly inhibit the production of IL-1β, IL-6, IL-8 and PGE2 induced by the PMA and the calcium ionophore
A23187 in the in vitro co-culture model
used. Introduced in formulation, borage seed oil aminopropanediol amides significantly
reduced the clinical manifestations of psoriasis and atopic dermatitis. Conclusion:
Our in vitro and in vivo study clearly showed the anti-inflammatory activity of borage
seed oil aminopropanediol amides and emphasized the putative role of pro-resolving
lipid mediators in the treatment of atopic dermatitis, psoriasis or other inflammation-induced
skin diseases.