TITLE:
Inhibitory Effect of α-Pinene on SGC-7901 Cell Proliferation and the Mechanism of ATM Kinase Signaling Pathway
AUTHORS:
Fuhong Zhu, Fengxiang Wei, Cui Zhang
KEYWORDS:
α-Pinene, SGC-7901 Cells, ATM Kinase, Proliferation, Cell Cycle, DNA Damage
JOURNAL NAME:
Chinese Medicine,
Vol.6 No.1,
March
11,
2015
ABSTRACT: Objective: To research the inhibitory effect on SGC-7901 cells of α-pinene, and
the related mechanism of α-pinene.
Methods: Used the MTT method to detect inhibition rate and western blotting to
detect the influence on expression of ATM, Phos-S1981ATM, H2AX, γH2AX, CHK2 and p-CHK2, p53 and phos-p53
cell cycle related protein in SGC-7901 cells. Results: The research found α-pinene could inhibit the proliferation
of SGC-7901 cells observably in vitro,
and the inhibition rate assumes the dependence on concentration; and western
blotting results showed that, α-pinene
could activate phospho-ATM, increase the amount of γH2AX (p p p p p p > 0.05). Conclusions: α-pinene could inhibit the proliferation
of SGC-7901 cells, and by inducing ATM (Ataxia Telangiectasia-Mutated) kinase
signal pathway in DNA damage response, activating cell cycle checkpoint, making
the cell cycle arrest then exerts its anti-tumor effects.