A. M. Makeen, M. N. Normah, S. Dussert and M. M. Clyde, “Cryopreservation of Whole Seeds and Excised Embryonic Axes of Citrus suhuensis cv. Limau Langkat in Accordance to Their Desiccation Sensitivity,” Cryo Letters, Vol. 26, No. 4, 2005, pp. 259-268.

has been cited by the following article:

• TITLE: Influence of Desiccation Time on Survival and Regeneration of Embryonic Axes of Groundnut (Arachis hypogaea L.) Immersed in Liquid Nitrogen

  AUTHORS: M. M. Abdulmalik, I. S. Usman, J. D. Olarewaju, D. A. Aba

  KEYWORDS: Cryopreservation; Groundnut; Embryonic Axes; Desiccation

  JOURNAL NAME: American Journal of Plant Sciences, Vol.4 No.9, August 29, 2013

  ABSTRACT: Cryopreservation, the storage of biological materials in liquid nitrogen (LN), is a useful method for long term conservation of plant germplasm. This study was carried out with the objective of establishing an efficient desiccation technique for successful cryopreservation and recovery of embryonic axes of groundnut. Embryonic axes of four groundnut (Arachis hypogaea L.) genotypes were evaluated. The excised embryonic axes were dehydrated by air current of a laminar air flow cabinet for different duration (0, 1, 2, 3, 4 & 5 hrs) before being plunged in LN (-196℃) and held for 1 hr. Samples were thawed in water bath at 40℃for 2 min, thereafter cultured on MS medium supplemented with 15 mg/L BAP for recovery. Highest survival (96.67%-100%) and shoot formation (91.67%-96.67%) were obtained at an average moisture content of 17% after 4-5 hr desiccation. Among the genotypes evaluated, Samnut 22 and Samnut 23 recorded the highest survival and shoot formation. This technique therefore appears promising for cryopreservation of groundnut germplasm.