TITLE:
Immunoresponse to Allogeneic Synovial or Xenogenic Mesenchymal Stromal Cells in a Co-Culture Model
AUTHORS:
Seth S. Jump, David S. Smith, David C. Flanigan, Alicia L. Bertone
KEYWORDS:
Arthritis; Stem Cells; Allogeneic; Xenogeneic; Immunoresponse
JOURNAL NAME:
CellBio,
Vol.2 No.1,
April
2,
2013
ABSTRACT:
The purpose of our investigations was to
measure, in a co-culture condition, the immunoresponse to allogeneic or xenogenic
cells, selected as potential sources for cell therapy of arthritis. We
challenged human spleen-derived cells (hSpl) by three different mechanisms: 1)
exposure to donor allogeneic or xenogeneic cellular antigens; 2)
exposure to donor cells transduced with adenoviral antigens (Ad) and 3)
lipopolysaccharide (LPS), a known inflammatory immunostimulant. The
immunoresponse to allogeneic human synovial-derived mesenchymal stromal cells
alone or transduced with adenoviral green fluorescent protein (hSD-MSC or
hSD-MSC/GFP) or the immunoresponse to xenogeneic equine mesenchymal stromal
cells (eqMSC) or equine dermal fibroblasts (eqDFb), characterized by the
proportion of CD3+, CD4+, and CD8+ human
splenocytes (hSpl), was measured on Day 0 and Day 6 of co-culture by flow
cytometry. In culture with hSD-MSC, hSD-MSC/GFP, eqDFb, or eqMSC, the
proportion of CD3+ and CD8+ hSpl increased with time in
culture but not with exposure to cell alloor xeno-antigens. Both hSD-MSC and
hSD-MSC/GFP increased in number during culture and were not affected in
viability or proliferation by co-culture with allogeneic hSpl. In this in
vitro, primary exposure study, hSpl demonstrated a natural selection and
adaptation to a short-term cell culture environment, and that neither
allogeneic nor xenogeneic cell antigens incited a greater cellular immunoactivation
than co-cultured hSpl alone.