TITLE:
A Novel Spectrophotometric Method for Determination of Five 1,4-Dihydropyridine Drugs in Their Tablets and Capsules Using Vanillin Reagent
AUTHORS:
Mohamed A. El Hamd, Sayed M. Derayea, Osama Hassan Abdelmageed, Hassan F. Askal
KEYWORDS:
Spectrophotometric Method; 1, 4-DHP; Validated; Selective; Vanillin Reagent
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.3,
March
28,
2013
ABSTRACT:
A selective and new
spectrophotometric method is described for determination of five 1,4
dihydropyridine drugs (1,4- DHP); namely nifedipine (NIF), nicardipine (NIC),
nimodipine (NIM), felodipine (FEL) and amlodipine (AML). The method is based on
a coupling reaction between the cited drugs and vanillin reagent in acidic
condition. Under optimized conditions, the red coloured products were
measured at 500 nm for NIF, NIC, NIM and FEL or at 479 nm for AML. Molar absorptivities were ranged from
0.575 × 104 － 1.065 × 104 l·mol-1·cm-1, Beer’s law was obeyed at 5 - 70 μg/mL
concentration range and the limit of detection was ranged from 0.150 - 1.500 μg/mL. The
proposed method was successfully extended to pharmaceutical preparations tablets
and capsules and comparison by
Student’s t-test and variance ratio F-test showed no significant
difference.