TITLE:
Mitochondrial comparative proteomic analysis of sterile line and its maintain line of purple cytoplasmic rice (Oryza sativa)
AUTHORS:
Lei Wei, Zhenjiang Fei, Xiaozhi Wu, Hualin Dong, Peng Zhou, Jianhua Zhang
KEYWORDS:
ATP Synthase; Comparative Proteomic Analysis; Cytoplasmic Male Sterile (CMS); Ying xiang A; Ying xiang B
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.1 No.3,
August
13,
2010
ABSTRACT: CMS/Rf systems in rice (Oryza sativa) have long been exploited for hybrid breeding to enhance productivity. Ying xiang CMS/Rf system is a new type. In this study, a mitochondrial comparative proteomic analysis of Ying xiang Sterile Line and its Maintain Line was started for a comprehensive investigation of the mitochondrial proteins’ functions in rice cytoplasmic male sterility. Mitochondria were prepared from rice shoots grown in the dark. Proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF/ MS. Using Mascot, it was found that 7 proteins were not described previously for plant mitochondria, indicating novel mitochondrial functions. 3 of them were characterized.