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has been cited by the following article:

• TITLE: Liver cytochrome P450 system as affected by endophyte-infected tall fescue seed extracts and ergot alkaloids

  AUTHORS: Ali S. Moubarak, Zelpha B. Johnson, Charles F. Rosenkrans Jr.

  KEYWORDS: Seed Extract; Tall Fescue; CYP3A4; Ergot Alkaloid

  JOURNAL NAME: Agricultural Sciences, Vol.3 No.1, January 5, 2012

  ABSTRACT: Endophyte infected tall fescue (E+) is the base diet for nearly all beef cattle in the southern USA. It has been linked to a variety of toxicological conditions due to the presence of large numbers of ergot alkaloids. This study was designed to investigate the effects of E+ seed extract and selected ergot alkaloids on the detoxification pathway by cytochrome P450 (CYP3A4) enzyme system. Tests were performed using the P450- Glo CYP3A4 enzyme activity kit (Promega, WI), according to the manufacturer’s manual. Luminescence was measured using a single tube TD20/20 luminometer. Endophyte infected tall fescue seed was extracted with 50/50 methanol/25 mM ammonium carbonate, cleaned and concentrated on Strata-X reversed phase column (Phenomenex). The extracts were evaluated on an HPLC, and then tested using a serial dilution method. Commercially available ergonovine (EN), ergocorine (ER), bromocryptine (BC) and ergocryptine (EC) were tested individually using 0 to 44 nM concentrations. Seed extract of E+ produced a significant (P P