TITLE:
Identification of Rhizobia Isolated from Nodules of Mexican Commercial Soybean Varieties
AUTHORS:
Cecilia Vázquez Rodríguez, Lourdes Vital López, Jesús Gerardo García Olivares, Homar Rene Gill Langarica
KEYWORDS:
Nodules, Soybean, Housekeeping Genes, MLSA, Rhizobia, Bradyrhizobium, Nitrogen Fixation, Symbiosis, Phylogenetic Analysis
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.15 No.1,
January
22,
2024
ABSTRACT: Rhizobia, crucial for nitrogen fixation in leguminous plants, play a
vital role in soybean cultivation. This study, conducted in Mexico, a major
soybean importer, aimed to identify bacteria from nodules of five soybean
varieties in high-production regions.
Multilocus sequence analysis (MLSA) was employed for enhanced species
resolution. The study identified six Bradyrhizobium species: Bradyrhizobium japonicum USDA 110, Bradyrhizobium japonicum USDA 6, Bradyrhizobium elkanii USDA
76, Bradyrhizobium neotropicale, Bradyrhizobium lablabi, and Bradyrhizobium icense. Bradyrhizobium japonicum USDA 110
predominated in the soils, displaying symbiotic preference for the Huasteca 400
variety. However, phylogenetic analysis didn't reveal a clear association
between strains, soil, and soybean variety. This research sheds light on the
diversity of rhizobia in Mexican soybean cultivation, contributing to the
understanding of symbiotic relationships in soybean production systems.