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has been cited by the following article:

• TITLE: The Effect of Increasing mTOR Signaling on the Speed of Regeneration in D. dorotocephala planaria

  AUTHORS: Ammar Danish

  KEYWORDS: Muscle Regeneration, Muscle Strains, mTOR Pathway, Leucine, Arginine

  JOURNAL NAME: Open Journal of Regenerative Medicine, Vol.12 No.3, September 7, 2023

  ABSTRACT: Muscle strains are a common injury that can occur during physical activity or exercise, and they can range from mild to severe depending on the extent of the damage. The mTOR pathway is a highly conserved signaling pathway that has been implicated in various cellular processes, including tissue regeneration. Previous studies that have investigated protein synthesis in mice have concluded that the mTOR pathway can improve muscle regeneration. However, the specific effects of mTOR pathway activation on muscle regeneration in planarians have yet to be fully explored. Therefore, this study aimed to investigate the use of Arginine and Leucine to stimulate the mTOR pathway for muscle strains in planarians. During the experiment, planarians were amputated, and different dosages of Arginine and Leucine were used to stimulate the mTOR pathway in Dugesia dorotocephala. The speed of muscle regeneration was measured over 14 days in five groups, with thirty planarians in each group. The results showed that the rate of regeneration from 0.134 mM Leucine solution showed a significant increase compared to the control group, while the groups exposed to 0.1, 0.3 mM Arginine, and 0.0134 Leucine did not show significant changes in muscle regeneration. These findings suggest that mTOR pathway activation may enhance muscle regeneration in planarians and that the effects may be dose-dependent. These findings have important implications for developing new treatments for tissue damage. Further studies are needed to fully understand the mechanisms of mTOR pathway activation on muscle regeneration in planarians and its potential use in tissue engineering and regenerative medicine.