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has been cited by the following article:

• TITLE: Possible Nature of an Electron Dense Substance in the Thylakoid Lumen of Chloroplasts

  AUTHORS: Galina Semenova

  KEYWORDS: Tanacetum vulgare L., Intrathylakoid Electron Dense Substance, Glutar Chlo-roplasts, Ultrastructure, TLC of Lipids

  JOURNAL NAME: CellBio, Vol.12 No.2, September 2, 2023

  ABSTRACT: The fixation of leaves of Tanacetum vulgare L. in glutaraldehyde makes it possible to isolate chloroplasts without sacrificing an electron dense substance in the thylakoid lumen. The extraction of lipids from the chloroplasts isolated from the leaves preliminarily fixed in glutaraldehyde has demonstrated that glycerolipids (galactolipids and phospholipids) are not manifested in TLC, whereas isoprenoid lipids (chlorophyll, carotenoids) are manifested. Presumably, isoprenoid lipids are not fixed with glutaraldehyde and are extracted from the thylakoid membrane. The ultrastructural control demonstrates that the electron dense substance from the thylakoid lumen is also extracted. It is possible that this substance is of isoprenoid nature.