TITLE:
Effect of Mancozeb Inhalation on the Olfactory Mucosa in Rats
AUTHORS:
Berzan Haznedar, Engin Deveci, Ertuğrul Gök
KEYWORDS:
Mancozeb, Olfactory Mucosa, IL-6, Rat
JOURNAL NAME:
International Journal of Otolaryngology and Head & Neck Surgery,
Vol.11 No.5,
August
25,
2022
ABSTRACT: Background: Mancozeb, (ethylene-bis-dithiocarbamate), is an
important fungicide useful against a wide range of fungus affecting ornamental
plants, crops, and fruits. We aimed to evaluate the changes in the olfactory
mucosa due to mancozeb toxicity and, if cytokine is active, IL-6
immunoactivity. Material and
Method: In experimental group, the mancozeb (500 mg/kg) was
administered with inhalation to 10 male Wistar Albino rats for five days a
week. The control group (n = 10) received distilled water with spray at the same
time period. The experiment was terminated after three weeks. Samples were placed in 10% formaldehyde for fixation
and placed in paraffin, sections of 5 μm were prepared from paraffin blocks and
stained with Hematoxylin-Eosin. Interleukin-6 (IL-6) primary antibody were
used for immunohistochemical analysis. Result: In the mancozeb group, olfactory epithelial cell
degeneration and apoptosis, inflammation, dilatation and congestion in the
vessels were observed. IL-6 expression was increased in vascular endothelium
and inflammatory cells. Conclusions: Mancozeb was thought that the increase in IL-6 expression due to the increase
in cell degeneration signal was thought to affect the development of cell
apoptosis and angiogenesis, and that the use of mancozeb might adversely affect
the olfactory mechanism.