TITLE:
Characterization and Quantification of Phenolic Compounds of Hydroethanolic Extracts and Fractions of Leaves Gnetum africanum (Welv.) and Gnetum buchholzianum (Engl.) (Gnetaceae)
AUTHORS:
Bertin Sone Enone, Gisèle Marie-Marguerite Etame-Loe, Charles Christian Ngoule, Jean-Pierre Ngene, Juliette Koube, Lahngong Methodius Shinyuy, Simone Véronique Fannang, Moïse Henri Julien Nko’o, Jean Baptiste Hzounda Fokou, Hans Denis Bamal, Celestine Magne, Valdi Steve Djova, Wilfred Ndogmo Tekapi, Sandrine Sikadeu, Josiane Etang, Albert Mouelle Sone
KEYWORDS:
Gnetum africanum, Gnetum buchholzianum, Phenolic Compounds, Liquid Chromatography, Mass Spectroscopy
JOURNAL NAME:
Open Journal of Applied Sciences,
Vol.12 No.7,
July
28,
2022
ABSTRACT: Research
for bioactive molecules and not resistant to infectious agents remains topical
for science. The total polyphenols (TPP) and total flavonoids (TFv) of the
hydroethanolic extracts and fractions obtained were quantified according to the
spectrophotometric method described in the literature using the Folin-Ciocalteu
reagent and the colorless solutions of sodium nitrite 2.5% and aluminum
chloride 10% respectively. The characterization of chemical compounds was made
by coupling liquid chromatography (LC) and mass spectroscopy (MS). Thus, 15
chemical compounds were characterized, seven from Gnetum africanum (EBa)
and eight from Gnetum buchholzianum (EBb). The majority of which were
stilbens, such as Gnetupendin D, Dimethoxygnetulin, Methoxyparvifolol D,
Isorhaponcitin from Eba and Gnetuhaidin P, Gnetupendin D, Gnetuhainin C,
phenols (Alcohol Homovanillyl, Alcohol
Erythro guaiacylglycerol-β-O-4'-coniferyl, and Alcohol Homovanillyl from
EBb. The minority were flavonoids such as Dimethoxydihydropyraneriodictyol, Dimethoxyeriodictyol
(EBa) and Dimethoxydihydropyraneriodictyol, Trimethoxyluteolin (EBb). The
levels of total polyphenolics and total flavonoids content were respectively 14.520
± 0.557 mg EAA/g Extract and 29.870 ± 2.485 mg EQ/g Extract for EBa. The levels
of total polyphenolics and total flavonoids content for EBb were respectively 4.560
± 0.010 mg EAA/g Extract and 2.233 ± 0.251 mg EQ/g Extract. The high levels of
flavonoids content respectively 220.200 ± 35.500 and 335.300 ± 57.810 mg EQ/g
Extract were shown in Dichlorometan fraction from EBa and EBb. These bioactive
compounds identified and total phenolics compounds quantified can be exploited
in pharmacological and pharmacokinetical activities to fight against
multi-resistant infectious agents and as well as oxidative stress and
antiinflammatory diseases.