TITLE:
Identified Analytical Profile for Microelements, Trace Metals, Amino Acids and Screening of Anti-Diabetic Activity from Flower and Leaf Extracts of Moringa oleifera in Streptozotocin (STZ)-Induced Diabetic Rats
AUTHORS:
Hemalatha Kamurthy, Sirajunisa Talath
KEYWORDS:
Moringa oleifera, Microelements, Nutritional Values, Gallic Acid, Amino Acids, Streptozotocin, Glibenclamide
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.13 No.6,
June
24,
2022
ABSTRACT: Background: Moringa
oleifera plant is popularly known for its rich phytoconstituents and
nutritional value and important medicinal values in both traditional and modern systems of medicine. We explored the present study for measurements
of microelements, amino acid, phenolic content in hydro-al-coholic flower and leaf extracts of Moringa oleifera along with anti-diabetic
activity in Streptozotocin (STZ)-induced diabetic male Wistar rats. Methodology: The micronutrients were determined by using atomic
absorption spectrophotometer at 285 nm and 422 nm for Calcium (Ca), phosphorus (P), Iron
(Fe), and Zinc (Zn), etc. The trace elements were also measured by spectrophotometer. The essential
amino acid was determined by using Amino acid analyser. The total phenolic
content in hydro-alcoholic extracts (flower and leaf) M. oleifera measured the absorbance at 760 nm by UV
spectrophotometer. The screening of anti-diabetic activity HAFE and HALE of Moringa oleifera at two different dose of 100 and 200 mg/kg b.w. for 21 days were performed by determining the changes in biochemical
parameters. Result and Discussion: The
results revels that the presence of micronutrients, trace elements and amino acids
in both flower and leaf of M. oleifera. The
hydrolaocholic extracts of HAFE and HALE at 200 mg/kg b.w. showed significant
antidiabetic activity compared with standard Glibenclamide. Whereas dose at 100 mg/kg b.w. showed moderate activity. Conclusion: In
conclusion, the M. oleifera exhibits
more effectiveness against STZ-induced diabetes. The HAFE and HALF extracts exhibited
significant anti-diabetic property and active components may be isolated and
clinical studies is required for further evaluation. Because of the rich source of phytoconstients,
nutritional elements will be helpful in processed food products as dietary supplements especially for
malnutrition in children in the current era.