TITLE:
Evaluation of Subacute Toxicity of Hydroethanolic Extracts Combinations from Gnetum africanum (Welv.) and Gnetum buchholzianum (Engl.) (Genetaceae) Leaves: Two Botanical with Antiproliferative and Antioxidant Potential
AUTHORS:
Bertin Sone Enone, Jean-Pierre Ngene, Angèle Foyet Fondjo, Charles Christian Ngoule, Loé Gisèle Etame, Loick Pradel Kojom Foko, Christophe Manz Koule, François Siewe, Ronald Bidingha Goufani, Josiane Etang, Albert Mouelle Sone
KEYWORDS:
Gnetum africanum, Gnetum buchholzianum, Subacute Toxicity, Oxidative Stress, Albino Rats
JOURNAL NAME:
Open Journal of Applied Sciences,
Vol.12 No.6,
June
24,
2022
ABSTRACT: This study was conducted to assess the
toxic effect of hydroethanolic extract combinations of Gnetum africanum Welv.
leaves and Gnetum buchholzianum Engl. (Gnetaceae) in experimental rats
to test the validity of the treatment of liver disorders related to oxidative
stress. The Combinations (m/m) 50-50 for E2 and 75-25 for E3 of ethanol-water
extracts from plant leaves at the respective doses of 100, 200, and 400 mg/kg
of body weight were used for 32-day toxicity. They were obtained after
harvesting leaves, sorting, drying in the air cover for three weeks, and
grinding. The resulting powder was doubly macerated with 70% ethanol for 48
hours and filtered. The filtrate was concentrated with the Heidolph-brand
rotary rotavapor and each extract obtained was preserved. The administrations
were carried out by gavage to wistar, male and non-pregnant female albino rats.
In the end, the animals were sacrificed. The serum and organ homogenates were
obtained for biochemical, tissue, and histopathological analyses respectively.
The analyses revealed insignificant variations at the 5% probability threshold
of the weight growth of experimental animals. These variations were found to be
statistically significant at the same probability for biochemical and tissue
parameters based on the dose of plant extracts and compared to control animals.
Histopathological analysis of liver tissue showed leukodate infiltration that
indicates extract-induced inflammation of the hepatocytes at the 400 mg/kg dose
of body weight in females. However, this infiltration of the cells would have
improved the regeneration of hepatocytes justified by the normal rate of
transaminases. These results showed that combinations of hydroethanolic
extracts of G. africanum and G. buchholzianumare non-toxicand may
be potential candidates in the Cameroonian flora medicinal plant database shown
in the monitoring of oxidative stress-related diseases.