TITLE:
In Vitro Clonal Propagation from Juvenile and Different Explant Types of Two Edible Annonaceae Species: Annona muricata L. and Annona squamosa L.
AUTHORS:
Oumar Ba, Abraham Dieme, Amadou Lamine Ndoye, Mame Ourèye Sy
KEYWORDS:
Annona muricata, Annona squamosa, Juvenile Material, Micropropagation, 6-Benzylaminopurine, 6-Furfuryl Aminopurine, 1-Naphthaleneacetic Acid, Indole-3-Butyric Acid
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.12 No.12,
December
22,
2021
ABSTRACT: Annona muricata L. and Annona squamosa L. are tropical species whose fleshy fruit is edible. They offer real possibilities for socio-economic
use, particularly in the fields of medicine, nutrition, ecosystem conservation
and the poverty alleviation. This study was set up to evaluate different
methods of micropropagation from juvenile material for the regeneration of
these species. Thus, MS medium supplemented with [BAP 2 mg·L-1] i.e. M2 produced 2.87 newly formed shoots from the cotyledonary nodes of A. muricata.
For the terminal apices of A. squamosa, it was MMS medium supplemented
with [BAP 2 mg·L-1] i.e. MM2 that was most conducive to new shoot formation (3.12). The
addition of 0.1 and 0.2 mg·L-1 of NAA in the M2 medium,
made it possible to have the best elongations
and average number of nodes for the new shoots from cotyledonary nodes of A. muricata (9.11 cm for 5.62 nodes). With A. squamosa, MM7 medium [MMS + BAP 1 mg·L-1 + KIN 1 mg·L-1] resulted in an average length of 9.05 cm with 5.62 nodes on average for
the apical shoots. A 3-day rhizogenic induction period in the dark with [IBA 50
mg·L-1] and 2 g·L-1 of activated
charcoal gave a rooting rate of 66.67% for shoots originating from the
cotyledonary nodes of A. squamosa; while with vitroplants from
cotyledonary nodes of A. muricata, a better rooting rate (83.33%)
was obtained following a 5-day rhizogenic induction. After 30 days of
acclimatization, the survival rate reached 83.33% for plants from the tips of A. muricata,
whereas for A. squamosa, it was plants grown from cotyledonary nodes that had the
same survival rate.