Tsuzukibashi, O., Uchibori, S., Kobayashi, T., Umezawa, K., Mashimo, C., Nambu, T., Saito, M., Hashizume-Takizawa, T. and Ochiai, T. (2017) Isolation and Identification Methods of Rothia Species in Oral Cavities. Journal of Microbiological Methods, 134, 21-26.
https://doi.org/10.1016/j.mimet.2017.01.005

has been cited by the following article:

• TITLE: Health Indicator Bacteria That Is Useful for Risk Assessment of Peri-Implantitis

  AUTHORS: Hidenori Suzuki, Osamu Tsuzukibashi, Akira Fukatsu

  KEYWORDS: Peri-Implantitis, Multiplex PCR, Rothia aeria, Oral Cavity

  JOURNAL NAME: Open Journal of Stomatology, Vol.11 No.9, September 18, 2021

  ABSTRACT: We established a novel identification method for oral Rothia species using one-step multiplex PCR analysis to investigate whether the monitoring of oral Rothia species levels is useful for peri-implantitis risk assessment, and to examine the oxygen concentration that these organisms need for growth in vitro. The mean number and proportion of Rothia aeria in peri-implant sulcus fluid (PISF) samples was significantly higher in the healthy implant group than in the peri-implantitis group (P R. aeria under aerobic conditions vigorously grew compared with those under anaerobic conditions, and this organism grew only at the upper layer where high oxygen concentrations existed in a semi-liquid nutrient medium. Therefore, the monitoring of R. aeria levels may be suitable as an indicator of healthy peri-implant tissue conditions for the prevention of peri-implantitis.