TITLE:
In Vitro Propagation and Cytological Analysis of Boerhaavia diffusa L—An Important Medicinal Plant Species of Bangladesh
AUTHORS:
Sweety Majumder, Animesh Biswas, Mohammad Mahbubur Rahman
KEYWORDS:
Boerhaavia diffusa, Explant, Micropropagation, Chromosome, Karyotype
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.12 No.9,
September
15,
2021
ABSTRACT: An effective and consistent in vitro propagation technique for Boerhaavia diffusa L. was developed using different types of explants and media compositions.
Shoot apex and nodal explants of field grown plants were aseptically cultured
on Murashige and Skoog (MS) medium supplemented with different concentrations
and combinations of auxins (IAA and NAA) and cytokinins (BAP and Kn). The
maximum number of multiple shoot buds (6.65 ± 0.09) obtained from
nodal explants on MS medium containing 2.0 mg/l BAP + 0.5 mg/l IAA. Multiple
shoot buds underwent rapid elongation (4.24 ± 0.06 cm) on MS media fortified
with 3.0 mg/l BAP + 0.5 mg/l NAA. Half strength MS media supplemented with 1.0
mg/l IBA + 0.5 mg/l IAA was better for induction and proliferation (6.42 ± 0.07)
of roots and 75% of plantlets were successfully acclimatized to ex vitro condition, exhibiting a normal
development. Somatic chromosome number of in
vivo and in vitro grown plants were confirmed to be 2n = 26. Chromosome length ranged from 1.40 to 2.43 μm
in the mother plants and 1.34 to 2.31 μm for in vitro grown plants. The
total form percent (TF%) of mother and in
vitro grown plants was 42.91% and 41.16%, respectively. The karyotype formula of in vivo grown plants was 2n = 26 = 4Lsm + 6Msm + 2Mm + 14Sm, whereas that of the in vitro grown plants was 2n = 26 = 8Lsm + 4Msm + 2Mm + 12Sm. The frequency of the
chromosome having arm more than 2:1 was 0.08 for in vivo grown plants and 0.15 for in vitro raised plants. Thus, according to Stebbins classification (1971) the karyotype
of both plants falls into 2A symmetrical type.