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has been cited by the following article:

• TITLE: Effect of Flaxseed on Bile Tolerances of Lactobacillus acidophilus, Lactobacillus bulgaricus, and Streptococcus thermophilus

  AUTHORS: Meghana Theegala, René Adalberto Chigüila Arévalo, Valerie Viana, Douglas Olson, Kayanush Aryana

  KEYWORDS: Flaxseed, Bile tolerance, Lactobacillus acidophilus, Lactobacillus bulgaricus, Streptococcus thermophilus, Probiotic

  JOURNAL NAME: Food and Nutrition Sciences, Vol.12 No.7, July 13, 2021

  ABSTRACT: Consumption of flaxseed provides health benefits. Bile tolerance allows survival of probiotics in the intestinal tract. The objective was to determine whether or not flaxseed enhances bile tolerance of Lactobacillus acidophilus (L. acidophilus) LA-K, Lactobacillus delbruekii ssp. bulgaricus (L. bulgaricus) LB-12, and Streptococcus salivarius ssp. thermophilus (S. thermophilus) ST-M5. Control and experimental (62 g flaxseed/L) broths containing 0.3% oxgall were prepared for each culture, sterilized, cooled, inoculated, and plated for 8 h. Growth of each microorganism in both the control and experimental broths was evaluated by the slope of the regression line of its log count versus time after inoculation. Flaxseed significantly enhanced growth of L. acidophilus but not L. bulgaricus and S. thermophilus over 8 h compared to its corresponding control. Therefore, flaxseed improved the bile tolerance of L. acidophilus but not of S. thermophilus and L. bulgaricus.