TITLE:
Influence of Amino Acids on the Secondary Somatic Embryogenesis Proliferation Process of Moroccan Cork Oak (Quercus suber L.)
AUTHORS:
Naouar Ben Ali, Lamarti Ahmad
KEYWORDS:
Quercus suber L., Secondary Embryos, Amino Acids, Glutamine, Asparagine, Casein Hydrolysat
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.12 No.4,
April
22,
2021
ABSTRACT: The present study aims to explore the regeneration
potential of Moroccan cork oak through the secondary somatic embryogenesis
process. Particularly, we focus on the analysis of amino acids influence on the
quantity and quality of the regenerated
secondary embryos. The amino acids tested are: Glutamine, asparagine, arginine, tryptophane, methionine,
casein hydrolysate and urea. Each amino acid is added in the Margara
(N30K) medium at different concentrations
ranged between 10 and 500 mg/l. The results are collected after 2 months
of culture. First analysis shows that the glutamine and the casein hydrolysate gives a maximum number of somatic embryos,
clusters and pre-embryos newly formed on the clusters. By comparison to
the control medium, the increase of the
secondary embryos number directly formed exceed 36% in the case of casein hydrolysate and 35% of in the case of glutamine both at the
concentration of 30 mg/l. However, the test of the combination of these amino
acids did not have any significant results. In terms of quality, the influence
of amino acids on the morphology of secondary embryos was analyzed.