TITLE:
Production of Plant Growth-Promoting Bacteria Biofertilizer from Organic Waste Material and Evaluation of Its Performance on the Growth of Corn (Zea mays)
AUTHORS:
Christie Oby Onyia, Amarachi Maryann Okoh, Okoh Irene
KEYWORDS:
Biofertilizer, Zea mays, NPK, Bacillus sp., Azotobacter sp., PGPB
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.11 No.2,
February
21,
2020
ABSTRACT: Biofertilizer can be defined as preparation that contains microbes capable ofnitrogen (N)-fixation and phosphate solubilization that promote plantgrowth. These groups of microbes, classified as Plant Growth-Promoting Bacteria (PGPB), colonize the rhizosphere and the soil. In this work, liquid biofertilizer was produced from whole orange, banana and grape, wheat and rice chaff, Moringa oleifera leaves, soil, and brown sugar (as carbon source) mixed with water and cultured in an anaerobic condition for two weeks. The sieved culture was stored in a tightly sealed PVC container at room temperature for biochemical analysis of microbial population. Nitrogen fixing bacteria (Azotobacter sp.) and phosphorus solubilizing bacteria were isolated using Ashby’s Mannitol Azotobacter medium and Pisvikoya’s PSB medium respectively, while Bacillus sp. was isolated using Bacillus agar. Field experiment was carried out to investigate the performance rates of the biofertilizer against those of the Nitrogen/Phosphorus/Potassium (NPK) chemical fertilizer and the control, on the growth of corn (Zea mays). The experimental design consisted of three treatments of the Biofertilizer, Chemical fertilizer (NPK) and Control, conducted in three replicates. Data collected were analyzed usingone-way ANOVA at P