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has been cited by the following article:

• TITLE: Over-Expression of MicroRNA-22 Protects Cardiomyocytes from Ischemic Injury

  AUTHORS: Lin Ling, Zicheng Ling, Shaohua Gu

  KEYWORDS: MicroRNA-22, Cardiomyocyte, Cell Activities

  JOURNAL NAME: World Journal of Cardiovascular Diseases, Vol.9 No.10, October 18, 2019

  ABSTRACT: Objective: To investigate the protection effect of over-expressed MicroRNA- 22 on ischemic cardiomyocyte.Methods: Cardiomyocytes cultured in hypoxia and ischemia condition were transfected with recombinant adenovirus vector containing microRNA-22 plus a recombinant green fluorescent protein (GFP). Transduction efficiency and expression of microRNA-22 were assessed. The expression of downstream PTEN protein expression was detected. After successful transduction, cell activities including viability, nuclei proliferation and cell apoptosis were assessed. We used CCK-8 assay to assess cell activities, EdU assay to assess nuclei proliferation and Caspase-3/LDH assay to assess cell apoptosis. Results: MicroRNA-22 was transfected successfully using adeno-virus. The expression of microRNA-22 was significantlyincreased. The transduction efficiency reached more than 95%. Over-expressionof microRNA-22 incardiomyocytes down regulated PTEN protein level.Over- expression of microRNA-22 increased cell activities, DNA proliferation and decreased cell apoptosis. Conclusion: Over-expression of microRNA-22 pro- tected cardiomyocytes from ischemic injury.