TITLE:
In Search of Regulators of LeSPL-CNR by South-Western Blotting and Yeast One-Hybrid Library Screening System
AUTHORS:
Xiaohong Wang, Bishun Ye, Ying Wang, Ting Zhou, Tongfei Lai
KEYWORDS:
Solanum lycopersicum, LeSPL-CNR, 286 bp Region, South-Western Blotting, Yeast One-Hybrid
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.9 No.5,
April
20,
2018
ABSTRACT:
LeSPL-CNR is a crucial transcription factor for fruit ripening of Solanum lycopersicum. The cnr (colorless
non-ripening) epimutation resulted from hypermethylation in a 286 bp region
of LeSPL-CNR promoter inhibits normal
fruit ripening. In present study, potential regulators of LeSPL-CNR, which could
bind to the specific 286 bp region, were screened via south-western blotting
and yeast one-hybrid (Y1H) library screening system. Results indicated that a
total of 13 and 19 candidate proteins were acquired respectively, and both
ribulose-1,5-bisphosphate carboxylase/oxygenase and 40S ribosomal protein were
identified by two methods. These would provide some information for revealing
roles of DNA methylation and the regulatory mechanism for LeSPL-CNR.