TITLE:
Immune Augmentation of Injectable PLGA – Dextran (PLDEX) a Double Polymeric Microspheres as an Adjuvant for Hepatitis B Vaccine
AUTHORS:
Sivakumar Sivagurunathan Moni, Sukumaran Natarajapillai, Mohammed M. Safhi
KEYWORDS:
HBsAg; PLGA – Dextran Microspheres; Vaccine Delivery; Immunogenicity
JOURNAL NAME:
World Journal of Vaccines,
Vol.1 No.3,
August
24,
2011
ABSTRACT: A new method has been developed to prepare microspheres by blending PLGA and dextran polymers (PLDEX) using solvent evaporation technique. Recombinant hepatitis B vaccine (HBsAg) was incorporated in to the double polymeric system. The objective of this study was to investigate the feasibility of PLDEX polymeric microspheres as an adjuvant for hepatitis B vaccine (HBsAg). The present study demonstrates the immunogenicity profile of HBsAg encapsulated in PLDEX and compared their efficacy with alum adsorbed HBsAg. The single intramuscular injection of HBsAg loaded PLDEX microspheres in Wistar rats resulted satisfactory antibody titers. Based on in vivo findings PLDEX microspheres were able induce satisfactory immune response.