TITLE:
Isolation of Campylobacters from Intestinal Tract of Poultry in Northern Region of India
AUTHORS:
Chetana Vaishnavi, Meenakshi Singh, Prashant Kapoor
KEYWORDS:
Antibiotic Resistance, Campylobacter, Molecular Investigation, Phenotypic Identification, Poultry, Serotyping
JOURNAL NAME:
Advances in Microbiology,
Vol.5 No.12,
November
16,
2015
ABSTRACT: Campylobacter is one of the most common food-borne
bacterial enteropathogens. We planned to investigate the prevalence and
antibiotic resistogram of Campylobacter in poultry in and around Chandigarh. Poultry samples
(n = 127) were obtained from slaughter houses/retail outlets and cultured microaerophilically on Campylobacter media. The isolates were identified phenotypically and
by molecular investigation. Identification of specific genes to look for
resistance to nalidixic acid, ciprofloxacin, tetracyclin and streptomycin was
also done. Campylobacter was isolated from 57/127 (44.9%) of the
samples. The most frequent serotypes identified were B: 2, S: 27, Z5:
52 and Z7: 57. All culture isolates (100%) were reconfirmed as Campylobacter by 16S rRNA polymerase chain reaction. Molecular identification of isolates
revealed the presence of C. jejuni in 45 (79.0%), C. coli in
1 (1.8%) and co-infection of C. coli and C. jejuni in 11 (19.3%). No C. lari and C. upsaliensis were detected. Antibiogram
typing showed nalidixic acid resistance in 36.8%, ciprofloxacin resistance in
35.0% and 31.5% resistance for both streptomycin and tetracyclin. A high level
of Campylobacter prevalence was found
among the poultry with C. jejuni being the most commonly isolated
species. Resistance to major antibiotics among Campylobacter isolates from poultry was also very high. The study
of prevalence of Campylobacter in
poultry and its resistance to major antibiotics will help to plan risk burden
strategies throughout the food chain.