TITLE:
Investigation of in Vitro and in Vivo Metabolism of Schisandrin B from Schisandrae Fructus by Liquid Chromatography Coupled Electrospray Ionization Tandem Mass Spectrometry
AUTHORS:
Tianxiu Qian, Pou Kuan Leong, Kam Ming Ko, Wan Chan
KEYWORDS:
Schisandrin B, Metabolism, Disposition, UPLC-MS/MS
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.6 No.8,
August
24,
2015
ABSTRACT: Schisandrin B (Sch B) is one of the active dibenzocyclooctadiene lignans found in the Schisandrae
Fructus. Experimental studies have shown that Sch B possesses various pharmacological properties,
including anti-cancer, neuroprotective and nephroprotective activities. However, no detailed
information on its biotransformation was reported in the literature. Here, we investigated the in
vitro and in vivo metabolism of Sch B by using ultra-performance liquid chromatography coupled
with tandem mass spectrometry. In vitro study detected and identified one oxygenated metabolite.
Four metabolites were detected and identified from the in vivo study. The results indicated that
the metabolism of Sch B mainly involved the demethylation of methoxy groups, the opening of
five-member ring and the glucuronidation of metabolites in rats. The metabolites were identified
for the first time by MS/MS analyses.