TITLE:
Studies on Xyloglucanase during the Germination of Seeds of Tamarindus indica
AUTHORS:
K. R. Siddalinga Murthy, S. Kantharaju
KEYWORDS:
Tamarindus indica, Xyloglucan, Xyloglucanase, Tamarind Seed
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.2 No.4,
June
12,
2014
ABSTRACT:
Germinating seeds of Tamarindus indica
contain endo-β-1, 4-xyloglucanases which degrade tamarind xyloglucan, but not
carboxymethylcellulose (CMC). The xyloglucanases are isolated from the
germinating tamarind seeds using 50 mM acetate buffer, pH 5.5 containing 0.5 M
NaCl. The Km value is 0.667 g/liter and the enzyme is optimally active at pH
5.5 and stable between pH 4 - 6.5. The optimum temperature is 45?C and is quite
stable upto 50?C. The activity declined by 50% at 60?C and is completely
inactivated at 70?C. Highest xyloglucanase activity and specific activity are
observed on the 23rd day of germination. The polyacrylamide gel electrophoresis
(PAGE) indicated the presence of five isozymes of xyloglucanases which are
visualized by activity staining separately with congo red and grams iodine.
Isozyme 2 is the major xyloglucanase present throughout the germination period.