TITLE:
Controlling Osteogenic Differentiation through Nanoporous Alumina
AUTHORS:
Shiuli Pujari-Palmer, Thomas Lind, Wei Xia, Liping Tang, Marjam Karlsson Ott
KEYWORDS:
Nanotopography; Nanoporous Alumina; Osteogenic Differentiation; Marrow Stromal Cells
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.5 No.2,
March
31,
2014
ABSTRACT:
Nanotopographical features are found to have
significant effects on bone behavior. In the present study, nanoporous aluminas with different pore sizes (20, 100 and 200 nm in diameter), were evaluated for their osteoinductive and drug
eluting properties. W20-17 marrow stromal cells were seeded on nanoporous
alumina with and without the addition of BMP-2. Although cell proliferation was
not affected by pore size, osteogenic differentiation was 200 nm as compared to 20 and 100 nm pores induced higher alkaline phosphatase
activity (ALP) and osteocalcin expression levels, thus indicating osteoblastic
differentiation. Cell morphology revealed that cells cultured on 20 nm pores adopted a rounded shape, while larger
pores (200 nm) elicited an elongated morphology. Furthermore, ALP expression levels were
consistently higher on BMP-2 loaded nanoporous alumina surfaces compared to
unloaded surfaces, indicating that not only is nanoporous alumina
osteoinductive, but also has the potential to be used as a drug eluting bone-implant
coating.