TITLE:
Induced Production of Exoglucanase, and β-Glucosidase from Fungal Co-Culture of T. viride and G. lucidum
AUTHORS:
Tanzila Shahzadi, Zahid Anwar, Zafar Iqbal, Awais Anjum, Tahir Aqil, Bakhtawar , Arroha Afzal, Muhammad Kamran, Sajid Mehmood, Muhammad Irshad
KEYWORDS:
Process Optimization; SSF; Co-Culture; T. viride; G. lucidum
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.5,
April
2,
2014
ABSTRACT:
In the present study, a
co-culture technique was adopted with an aim to investigate a hyper production of exoglucanase, and β-glucosidase
using cheap and easily available agro-industrial residue corn stover as growth
supporting substrate. Various physio-chemical and nutritional variables were
optimized using classical and completely randomized designs for induced
production of exoglucanase, and β-glucosidase
from the co-culture of Trichoderma viride and Ganoderma lucidum in solid state
fermentation (SSF). Analysis profile showed that when the conditions of the SSF
medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with
6 mL of inoculum were optimal, the maximum productions of exoglucanase (485 ±
6.5 U/mL) and β-glucosidase (255 ±
3.3 U/mL) were recorded after 5 days of incubation at pH 6 and 35°C.