TITLE:
Detection of Helicobacter pylori in dental plaque of mexican children by real-time PCR
AUTHORS:
J. A. Valdez-Gonzalez, P. C. Mares-Moreno, M. J. Kowolik, J. Vargas-Villlarreal, F. Gonzalez-Salazar, M. A. De la Garza-Ramos
KEYWORDS:
Helicobacter pylori; Oral Pathology; Children; Real Time Polymerase Chain Reaction; Anaerobic Bacteria
JOURNAL NAME:
Health,
Vol.6 No.4,
February
18,
2014
ABSTRACT:
Dental plaque in adult patients is well identified as a
reservoir for Helicobacter pylori. This
question still remains unclear in children. The aim of this study is to
identify the presence of this bacterium in dental plaque of Mexican pediatric
patients, using Real Time Polymerase Chain
Reaction (qPCR). Forty patients from 2 to 11 years without dyspeptic
symptoms were enrolled. Samples were collected from the subgingival space of
the lingual side of the lower molars and cultured in selective medium. Therefore, qPCR analysis was conducted. According to
the results obtained in this study, it was found that 35% of the pediatric population who participated tested qPCR positive for the presence of H. pylori in dental plaque samples. No significant
associations were detected among isolation
rate by gender or age. We found that dental plaque may be a reservoir for H. pylori. However, more research is needed to establish the way of the
infection of pediatric population.