Article citationsMore>>
Maekawa, N., Hiramoto, M., Sakamoto, S., Azuma, M., Ito, T., Ikeda, M., Naitou, M., Acharya, H.P., Kobayashi, Y., Suematsu, M., Handa, H. and Imai, T. (2011) High-performance affinity purification for identification of 15-deoxy-Δ12,14-PGJ2 interacting factors using magnetic nanobeads. Biomedical Chromatography, 25, 466-471.
http://dx.doi.org/10.1093/nar/11.5.1475
has been cited by the following article:
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TITLE:
Prohibitins, novel vitamin K2 target factors in osteoblast
AUTHORS:
Tatsuya Uebi, Makoto Umeda, Naoya Maekawa, Satoshi Karasawa, Hiroshi Handa, Takeshi Imai
KEYWORDS:
Vitamin K2; Prohibitin; Osteoblast; Runt-Related Transcription Factor 2 (Runx2)
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.1 No.3,
December
12,
2013
ABSTRACT:
Vitamin K2 (VK2,
menaquinone) is a drug for osteoporosis.
VK2 acts as a cofactor for γ-glutamyl carboxylase, which catalyzes the
carboxylation of specific glutamic acid residues (γ-carboxylation)
of substrate proteins. Here we demonstrate that VK2 also regulate
osteoblastgenic marker gene expression. Using VK2-immobilzed nanobeads new target proteins
were purified and
identified from osteoblastic cell line. They are prohibitin 1 and 2 (PHB1 &
2), respectively. To confirm the PHBs function on VK2-dependent transcription, PHB1 & 2 were knock-down
and osteocalcin gene 2 transcriptions were analyzed, indicating that PHBs
regulate VK2-dependent transcription. Taken together PHBs are VK2 target
proteins for osteoblastgenic transcription.
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