TITLE:
Aqueous Two-Phase Systems Applied to Partition Proteins from Goat Milk Whey In-Nature
AUTHORS:
Luziany A. C. Freire, Camila G. Pereira
KEYWORDS:
Goat Milk Whey, α-Lactoalbumin, β-Lactoglobulin, Aqueous Biphasic Systems
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.7 No.9,
September
13,
2016
ABSTRACT: The proteins coming from the milk whey have numerous functional properties.
Among the proteins with high bioactivity, α-lactoalbumin (α-La) and β-lactoglobulin
(β-Lg) are present in large quantities in the milk whey. In the separation process of
proteins, it is important to choose techniques which besides ensuring purity and high
yield will not affect the molecule biological activity. The aqueous two-phase systems
(ATS) have been utilized with success in the partition of these proteins, however, the
studies were performed using protein in its pure form. Studies using milk whey
in-nature and goat milk whey have not been found yet. In this context, the objective
of this study was to evaluate the liquid liquid equilibrium of aqueous two-phase systems
(ATS) in the partition of α-La and β-Lg from goat milk whey in-nature. Equilibrium
data were performed considering ATS comprised of polyethylene glycol, potassium
phosphate and water at 25°C and pH 7.0. The influence of the polymer molecular
weight and amount of goat milk whey in-nature on the partition coefficient of
these proteins were assessed. The partition coefficient, selectivity, process yield and
purity of α-lactoalbumin and β-lactoglobulin proteins were determined. The results
showed that the separation technique by aqueous biphasic systems is applicable indicating
high efficiency in the whey proteins separation process.