TITLE:
Total RNA Degradation in Vitro and in Vivo by Glutamate Dehydrogenase-Synthesized RNA Enzyme: Biotechnological Applications
AUTHORS:
Godson O. Osuji, Wenceslaus C. Madu, Paul M. Johnson
KEYWORDS:
Arachis hypogaea, Limited Resource Farmers, Stoichiometric Salt Mixes, GDH Isoenzyme Purification, Nongenetic Code-Based RNA, Electrophoresis, DNA:RNA Hybrids
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.10 No.4,
April
15,
2019
ABSTRACT: Glutamate dehydrogenase regulates
crop development, growth, and biomass yield through its synthesis of
non-genetic code-based RNA. Understanding the mechanism of GDH-synthesized RNA
enzyme would enhance the agriculture
innovation capacity of the more than a billion urban gardeners, smallholder, and limited resources indigenous farmers. Different metabolic
variants were prepared by treating peanut growing on healthy soil with
stoichiometric mixes of mineral salt solutions. Peanut GDH charge isomers were
purified to homogeneity by electrophoresis, and made to synthesize RNA enzyme.
Peanut total RNA was 5’-end labeled with [γ-32P]ATP
and made to react as substrate in vitro with GDH-synthesized RNA from another metabolic variant of peanut. Agarose, and
polyacrylamide gel electrophoresis of the reaction products showed that tRNA,
rRNA, and most of the mRNAs were degraded to mononucleotides, but total RNAs
that were not mixed with GDH-synthesized RNAs were not degraded. When the
non-homologous sequence sections of the GDH-synthesized RNA were clipped out,
the homologous sections failed to produce Northern bands with peanut total RNA.
Therefore, the non-homologous sequence
sections served to identify, position, and align the GDH-synthesized RNA to its
target total RNA site independent of genetic code; the degradation of total RNA
being via non-canonical base alignments in the enzyme-substrate complex,
followed by electromagnetic destruction of the total RNA, the less stable of
the two kinds of RNA. This is the science-based corner stone that buttresses
the crop production efforts of limited resources farmers because
GDH-synthesized RNAs quickly degrade superfluous total RNA of the crop in
response to the soil mineral nutrient deficiencies thereby minimizing wastage
of metabolic energy in the synthesis of unnecessary protein enzymes while
optimizing biomass metabolism, crop growth, and maximum crop yields. In vitro hydrolysis of total RNA by
GDH-synthesized RNA is the game changing, prototype, R&D methods for
cleansing sick total RNA from cells, tissues, and whole organisms.