TITLE:
Safety Assessment and Potential Anti-Inflammatory Effect of Ethanolic Extract of Syzygium aromaticum in Albino Rats
AUTHORS:
Twadu A. Saeed, Osman A. Osman, Ahmed E. Amin, Samia M. A. El Badwi
KEYWORDS:
Syzygium aromaticum, Ethanolic Extract, Safety, Anti-Inflammatory, Rats
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.8 No.11,
November
16,
2017
ABSTRACT: The
present study was undertaken to investigate the effect of the ethanolic extract
of Syzygium
aromaticum to
albino rats. Forty eight Albino rats were employed to test the safety and the
anti-inflammatory effect of the extract. Safety of the extract was examined on
experimental animal’s model at three dose levels of the extract orally in daily
doses for three weeks. Effects of S.
aromaticum on rats revealed no significant effect on biochemical or haematological parameters.
The anti-inflammatory effect of the extract was tested in four equal groups; groups 1 and 2 were treated with 250 and
500 mg/kg of the extract, respectively, group 3 was treated with indomethacine and group 4 was the
untreated control. Carrageenan was used as an acute form inducer of inflammation. Indomethacine, the non-steroidal
anti-inflammatory drugs (NSAIDs), was used as a reference compound. Oedema size was
monitored at the 1st, 2nd, 4th, 6th and after 24 hours. The ethanolic
extract of S. aromaticum showed significant (P 0.001) decreased in the oedema size at efficacy rates of 79.41%, 82.39% and 63.92% for the dose, 500 mg/kg body weight at
the 2nd, 4th and 6th hour respectively higher
than that produced by indomethacine.