TITLE:
Decreased Ratios of Interleukin-1β and Intercellular Adhesion Molecule 1 in Gingival Crevicular Fluid after Scaling and Root Planing Associated with Periodontal Pocket Healing
AUTHORS:
Yuriko Hieda, Michihiko Usui, Taiji Nakamura, Masaki Morishita, Tomoya Hanatani, Yuki Moritani, Yukiko Koga, Shingo Kasai, Maki Inoue, Keisuke Nakashima
KEYWORDS:
IL-1β, ICAM-1, GCF, SRP, Wound Healing
JOURNAL NAME:
Open Journal of Stomatology,
Vol.7 No.9,
August
25,
2017
ABSTRACT: Objectives: Several researchers have investigated inflammatory
cytokines in gingival crevicular fluid (GCF) from chronic periodontitis with
clinical parameters after periodontal treatment. The aim of the present study
was to identify the inflammatory cytokines present in GCF associated with
periodontal pocket healing after scaling and root planing (SRP). Methods: Twenty patients were enrolled,
and clinical examinations including probing pocket depth (PPD) and bleeding on
probing (BOP) were performed at the first visit, and before and after SRP. The
periodontal epithelial surface area (PESA) and periodontal inflamed surface
area (PISA) were also calculated. GCF samples were collected before and after
SRP to measure the levels of 40 inflammatory cytokines using antibody array.
Correlations between changes in cytokine levels and clinical improvements were
assessed by single and multiple regression analyses. Results: PPD, PESA and PISA significantly decreased after SRP,
while there were no significant differences in the levels of cytokines before
(PreSRP) and one week after SRP (PostSRP). Clinical improvements (ΔPPD, ΔPESA
and ΔPISA) were calculated using the formula PreSRP-PostSRP, while changes in
the levels of inflammatory cytokines were calculated using three formulas: PreSRP-PostSRP,
PostSRP/PreSRP and Log(PostSRP/PreSRP). Log (PostSRP/PreSRP) for interleukin-1β (IL-1β) significantly correlated with
ΔPESA (R = -0.512). PostSRP/PreSRP and Log(PostSRP/PreSRP) for intercellular adhesion
molecule 1 (ICAM-1) significantly correlated with ΔPISA (R = -0.669, -0.573). Two good
fit models were generated by multiple linear regression. Log(PostSRP/PreSRP)
for IL-1β significantly affected
ΔPESA (R2 = 0.566).
PostSRP/PreSRP for ICAM-1 significantly affected ΔPISA (R2 = 0.615). Conclusion: Changes (PreSRP-PostSRP) in any cytokines in GCF after SRP were not associated
with clinical improvements. However, the present study indicates that lower
PostSRP/PreSRP ratios for IL-1β and
ICAM-1 in GCF lead to a greater decrease in PESA and PISA, respectively.
Periodontal pocket healing might be affected by a decreased ratio of
inflammatory cytokines such as IL-1β and ICAM-1 at an early stage after SRP.