TITLE:
Essential Oils from Mentha piperita, Cymbopogon citratus, Rosmarinus officinalis, Peumus boldus and Foeniculum vulgare: Inhibition of Phospholipase A2 and Cytotoxicity to Human Erythrocytes
AUTHORS:
Danubia Aparecida de Carvalho Selvati Rezende, Maria das Graças Cardoso, Rafaela Vieira Souza, Maria Luisa Teixeira, Rafaela Magalhães Brandão, Vanuzia Rodrigues Fernandes Ferreira, Jéssica Oliveira e Nogueira, Maisa Lamounier Magalhães, Silvana Marcussi, David Lee Nelson
KEYWORDS:
Natural Products, Snake Venoms, Hemolysis, Pharmacological Potential
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.9,
August
14,
2017
ABSTRACT: The essential oils from Mentha piperita, Cymbopogon
citratus, Rosmarinus
officinalis, Peumus
boldus and Foeniculum
vulgare were
extracted by hydrodistillation and characterized and quantified by GC-MS and
GC-DIC. The oils induced hemolysis with all the doses evaluated (0.6 to 1.8 μL), and the diameters of the halos varied between 9
and 15 mm. Pre-incubation of P.
boldus oil
with Bothrops
jararacussu venom
resulted in potentiation of venom-induced hemolysis (30%) (proteases and
phospholipases A2). The essential oil from M.
piperita (0.6 μL) inhibited venom-induced hemolysis by 45%, whereas
0.6 μL of R.
officinalis oil
increased the hemolysis by 20%. For the essential oil from F.
vulgare, 100%
inhibition of activity (0.6 and 1.2 μL) was observed. The application of C.
citratus oil
induced hemolysis with all the volumes evaluated. Phospholipase activity
induced by the venom was only inhibited (10%) with the 0.6 μL volume of R.
officinalis oil.
The oils from M.
piperita and F.
vulgare (1.8 μL) and C.
citratus oil
(0.6 μL)
potentiated the phospholipase activity. The results highlight the need for a
broad characterization and regulation of the use of natural products, because
they can have therapeutic or toxic actions.