Article citationsMore>>
Adams, P.D., Afonine, P.V., Bunkoczi, G., Chen, V.B., Davis, I.W., Echols, N., Headd, J.J., Hung, L.W., Kapral, G.J., Grosse-Kunstleve, R.W., McCoy, A.J., Moriarty, N.W., Oeffner, R., Read, R.J., Richardson, D.C., Richardson, J.S., Terwilliger, T.C. and Zwart, P.H. (2010) PHENIX: A Comprehensive Python-Based System for Macromolecular Structure Solution. Acta Crystallographica Section D, Biological Crystallography, 66, 213-221.
https://doi.org/10.1107/S0907444909052925
has been cited by the following article:
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TITLE:
Structural Basis for the Interaction of 14-3-3β with Tricarboxylic Acid Cycle Intermediate Malate
AUTHORS:
Zhiqiang Hou, Lijing Su, Xiaoyan Liu
KEYWORDS:
Crystal Structure, 14-3-3 Protein, ChREBP, Transcription Activation, Malate, Malic Acid
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.5 No.8,
August
3,
2017
ABSTRACT: The
protein family of 14-3-3(s) has risen to a position of higher importance as an
adaptor protein in cell biology. The seven highly conserved human 14-3-3
proteins coordinate diverse cellular processes including apoptosis, DNA damage
response, protein trafficking, and others. In liver hepatocytes, 14-3-3β binds to Ser196-phosphorilated
glucose-responsive carbohydrate response element-binding protein (ChREBP) to
inhibit converting excess carbohydrate to fat by regulating the nuclear/cytosol
trafficking of ChREBP. Here, we report X-ray crystal structures of homodimeric
mammalian 14-3-3β in its apo,
Malate-bound forms. The determined apo structure was captured with one monomer
in the closed state, whereas the other one had an open conformation.
Strikingly, 14-3-3β binds Malate
dynamically with a double-closed state, which is distinct from all previously characterized 14-3-3(s) and target ligand-binding modes. Malate docks into a first-time
observed cofactor pocket located at the concaved interface of 14-3-3β helices α2, α3, α4 through mainly electrostatic and hydrogen
interactions. Such a Tricarboxylic Acid Cycle intermediate Malate bond model
might offer a new approach to further analyze insulin-independent 14-3-3/ChREBP
pathway of de novo fat synthesis in
the liver.
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