TITLE:
Microtopography Attenuates Endothelial Cell Proliferation by Regulating MicroRNAs
AUTHORS:
Dan Wang, Mengya Liu, Shuangying Gu, Yue Zhou, Song Li
KEYWORDS:
Microgroove, PDMS, MicroRNA, Endothelial Cell
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.8 No.3,
July
14,
2017
ABSTRACT: Endothelial cell (EC) morphology can be regulated by
the micro/nano topography in engineered vascular grafts and by hemodynamic
forces in the native blood vessels. However, how EC morphology affects miRNA
and thus EC functions is not well understood. In this study, we addressed this
question by using human umbilical vein endothelial cells (HUVECs) cultured on
microgrooves as a model. HUVECs were grown on either microgrooved (with 10 μm
width/spacing and 3 μm depth) or smooth surfaces. HUVECs on microgrooved
surface had elongated and bipolar morphology, while HUVECs on smooth surface
showed cobble stone shape or non-polar morphology. EdU staining indicated that
HUVECs with elongated morphology had lower proliferation rate compared to their
counterpart cultured on smooth surface. Quantitative PCR analysis demonstrated
that the expression of the specific microRNAs (miR-10a, miR-19a, miR-221) that
targeted proliferation-related genes was all up-regulated. Consistently, the
mRNA levels of their respective target genes, mitogen-activated protein kinase
kinase kinase 7, Cyclin D1 and c-kit were significantly reduced by a fold
change of 0.12 ± 0.01 (p p 0.05) and 0.76 ±
0.21 (p