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https://doi.org/10.1371/journal.pone.0004760

has been cited by the following article:

• TITLE: Developing Stable Cultivar through Microspore Mutagenesis in ×Brassicoraphanus koranhort, Inter-Generic Allopolyploid between Brassica rapa and Raphanus sativus

  AUTHORS: Soo-Seong Lee, Byoung Ho Hwang, Tae Yoon Kim, Jeongmin Yang, Na Rae Han, Jongkee Kim, Hyun Hee Kim, Hadassah Roa Belandres

  KEYWORDS: xBrassicoraphanus, Intergeneric Hybrid, Baemoochae, Brassica rapa, Raphanus sativus

  JOURNAL NAME: American Journal of Plant Sciences, Vol.8 No.6, May 26, 2017

  ABSTRACT: A stable progeny was developed through induced mutation, using microspore culture, of the hybrid (F1F1) produced by crossing a newly synthesized, unstable allopolyploid (F1) and a stable cultivar, BB#1(F1) in xBrassicoraphanus. An F1F1 plant was subjected to the induced mutation system established during production of BB#1. Morphological characteristics of the progeny such as color, and leaf number and length, differed from those of BB#1. The bolting time of the progeny in spring cropping was very late compared to BB#1, allowing it to be grown to an adult plant in spring. Genomic in situ hybridization analysis of pollen mother cells at prophase I identified 19 bivalents, 10 from Brassica rapa and 9 from Raphanus sativus. The glucoraphenin content was almost identical to that of BB#1. Two cultivars are available in the baemoochae crop now. These results indicate that induced mutation using microspore culture is a viable method of stabilizing intergeneric allopolyploids between B. rapa and R. sativus.