TITLE:
Alterations in Low-Z Elements Distribution in Heart Tissue after Treatments to Breast Cancer Using LEXRF Technique
AUTHORS:
Andrea Mantuano, Regina Cely Barroso, Liebert Parreiras Nogueira, Marcos Vinicius Colaço, Carla Lemos Mota, Arissa Pickler, Delson Braz, Camila Salata, Samara Ferreira-Machado, Carlos Eduardo de Almeida, Alessandra Gianoncelli
KEYWORDS:
Micro-X-Ray Fluorescence, Low-Z Elements Distribution, Myocardial, Cardiac Tissue
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.7 No.11,
October
31,
2016
ABSTRACT: Data from Global Cancer Statistics show that breast cancer (BC) is the most common type of cancer among women, leading the number of deaths caused by cancer. The developments in diagnosis and treatment techniques for the BC, including chemotherapy and/or radiotherapy, increased the survival rates for this type of cancer. One late complication induced by BC treatment is the cardiotoxicity. This term comprises different cardiotoxic side effects, which include blood pressure alterations, myocardial ischemia, congestive heart failure and other damages. This study aimed to evaluate the cardiac alterations induced by radiotherapy and chemotherapy, simulating a treatment for BC in Wistar rats. It is, therefore, important to understand the mechanisms involved in the cardiotoxicity, in order to prevent women from this late effect, when they undergo BC treatments. The major interests in this work are in Low atomic weight elements as Sodium, because it is strongly related to cardiomyocyte contraction; Magnesium, because it is important in the cardiac metabolism; and Iron, because BC treatment induced cardiotoxicity can be associated to the oxidative stress. Changes that occur in unhealthy tissues in case to cardiovascular damages can be better understood when elemental compounds and structures of healthy tissues are known. Low Energy X-ray Fluorescence (LEXRF) technique was used to obtain elemental maps of low Z-elements providing a semi-quantitative analysis of the tissues evaluated under different conditions. Through the technique LEXRF we obtained elemental and absorption maps. The results showed more damages when associating chemotherapy and radiotherapy in comparison to myocardium healthy. Those images taken together with light microscopy, X-ray absorption and phase contrast images, satisfactorily characterize the cardiac tissue for the first time in the literature, from the structural and morphological points of view. LEXRF was carried out at TwinMic beamline in the ELETTRA Synchrotron Fa-cility, at the beamline TwinMic, in Trieste, Italy.