TITLE:
Induced Differentiation of Epithelioid Carcinoma Cell Lines: Evidence for Tumor Cell Quantal Mitosis
AUTHORS:
John J. Wille, Jong Y. Park
KEYWORDS:
Cell Differentiation, Epidermoid Carcinoma Cell Lines, A431, Hela, Kb, SCC-25 Keratinocytes, Cytochemical Staining Methods, Quantal Mitosis
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.7 No.11,
October
31,
2016
ABSTRACT: The
effects of growth factors and calcium concentrations present in different culture media
on induction of terminal differentiation were investigated for four different
epidermoid carcinoma cell lines, Hela, KB, A431, and SCC-25, and
their responses determined relative to those elicited by normal human keratinocytes
subjected to these culture conditions. Differentiation status was determined
cyto-chemically by a validated keratin protein staining method, and by
autoradiographic analyses. Growth and
differentiation promoting factors that influenced the direction of integrated control of growth and differentiation in normal human keratinocytes were found
to be effective for some cell lines but not others. The factors examined were
1) high density arrest in serum-free and serum-containing media, 2) media
shifts from high density culture in serum-containing media to low density
growth factor-depleted or supplemented serum-free medium, and 3) the
concentration of calcium in the media. The
extent and degree of differentiation achieved varied among different
cell lines depend on the presence or absence of serum, EGF and insulin protein
growth factors. Certain growth media appear
to sponsor keratin protein, cyto-chemically-detected differentiation, and
evidence of quantal mitotic division in low density HeLa cell and SCC25 cell cultures.
Epidermoid carcinoma cell lines retain limited capacity to commit to early
stages of cell differentiation.