TITLE:
Analysis of Some Important Forage Quality Attributes of Southeastern Wildrye (Elymus glabriflorus) Using Near-Infrared Reflectance Spectroscopy
AUTHORS:
Jason Brett Rushing, Uttam K. Saha, Rocky Lemus, Leticia Sonon, Brian S. Baldwin
KEYWORDS:
Acid Detergent Fiber, Digestible Dry Matter, Dry Matter Intake, Crude Protein, Near-Infrared Spectroscopy, Neutral Detergent Fiber, Wildrye
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.7 No.9,
September
28,
2016
ABSTRACT: Southeastern
wildrye (Elymus
glabriflorus, Vasey ex L.H. Dewey) is a
cool-season, perennial grass native to southeastern United States. Recently,
there is a growing interest in its development as a grazing and haying forage
crop due to its wide area of adaptation across this region. Consequently, there
is a great need for the evaluation of its forage quality by rapid, but accurate
analytical methods like Near-Infrared Reflectance Spectroscopy (NIRS). In this
study, acceptable NIRS calibration models were developed for: dry matter,
DM (n = 113, R2 = 0.904, RSCD = 2.54,
RSCIQ = 4.65); crude protein, CP (n = 113, R2 = 0.974, RSCD = 5.16,
RSCIQ = 5.92); acid detergent fiber, ADF (n = 116, R2 = 0.896, RSCD = 2.35, RSCIQ = 1.28); neutral detergent fiber, NDF (n =
118, R2 = 0.934, RSCD = 2.53, RSCIQ = 3.38); digestible dry matter, DDM (n = 116, R2 =
0.895, RSCD = 2.36, RSCIQ = 1.35); dry matter intake, DMI (n = 115, R2 = 0.924, RSCD = 2.40, RSCIQ = 2.53); and relative feed value, RFV (n = 114, R2 = 0.932, RSCD = 2.94, RSCIQ = 2.81). Prediction of independent validation
sets yielded good agreement between the NIRS predicted values and the
laboratory reference values for each of: DM (n = 53, R2 = 0.831, RPD
= 2.45, RPIQ = 4.24); CP (n = 57, R2 = 0.967, RPD = 5.37,
RPIQ = 7.16); ADF (n = 49, R2 = 0.895, RPD = 2.97,
RPIQ = 1.51); NDF (n = 53, R2 = 0.928, RPD = 3.75, RPIQ =
4.22); digestible dry matter, DDM (n = 55, R2 = 0.860, RSCD = 265,
RSCIQ = 1.15); dry matter intake, DMI (n = 156, R2 = 0.845, RSCD =
2.48, RSCIQ = 2.11); and relative feed value, RFV (n = 55, R2 =
0.916, RSCD = 3.45, RSCIQ = 3.04) contents, indicating that all seven
calibration models had good quantitative information. Therefore,
precise, accurate, and rapid analysis of these important forage quality
attributes of southeastern wildrye can be routinely done using the developed
NIRS calibration models.