Chen, R., Jin, Y.H., Li, Q.L., Chen, X.L., Yang, Y.S. and Wu, H. (2014) Some Effec-tive Methods for Dealing with the Problem of Hyperhydricity in Cloning Purple Coneflower. 2013 International Conference on Biological, Medical and Chemical Engineering (BMCE2013), DEStech Publications, Inc., 319.

has been cited by the following article:

• TITLE: Mannitol and Sorbitol Improve Uniformity of Adventitious Shoots Regeneration in Echinacea purpurea L. Moench

  AUTHORS: Xiaolu Chen, Dongliang Li, Yuesheng Yang, Hong Wu

  KEYWORDS: Echinacea purpurea, Micropropagation, Tissue Culture, Mannitol, Sorbitol, Osmotic Pressure

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.9 No.10B, September 23, 2016

  ABSTRACT: Mannitol or sorbitol was added into the Murashige and Skoog (MS) medium contain-ing certain concentrations of 6-Benzyladenine (BA) which was used to induce adventi-tious buds of Echinacea purpurea L. Results showed that the induced adventitious buds growing from medium added with 15 g·L-1 mannitol or sorbitol of the same con-centration were more consistent in height. The regeneration rates in MS medium containing 0.2 mg·L-1 BA and 15 g·L-1 mannitol were increased, while in MS medium containing 0.2 and 0.5 mg·L-1 BA, and 15 g·L-1 sorbitol, the regeneration rates were suppressed. On the other hand, genotype of explants and the concentration of BA in-fluenced the incidence of hyperhydricity, and the hyperhydricity of regenerated buds was more severe when the petiole explants were inoculated on medium with 15 g·L-1 mannitol or 15 g·L-1 sorbitol. The present study offers new possibility to the production of uniform plantlets for commercial cultivation in this important medicinal plant.