TITLE:
The Influence of HIF-1α Expression on Apoptosis and Number of T Lymphocyte in Peyer’s Patches after Burn with Delayed Fluid Resuscitation in Rats at Plateau
AUTHORS:
Cheng Zhang, Yi Liu, Ming Ma, Shifan Zhang
KEYWORDS:
Delayed Fluid Resuscitation, Peyer’s Patches, Apoptosis, HIF-1α, Burn, Plateau
JOURNAL NAME:
Surgical Science,
Vol.7 No.9,
September
13,
2016
ABSTRACT: Objective:
To research the expression of hypoxia inducible factor-1 alpha (HIF-1 alpha) on
the apoptosis and number of T lymphocyte in Peyer’s patches after severe burn
on plateau in rats. Methods: Wistar rats (n = 130) were subjected to deep
thickness burn injury (30% TBSA, III degree), at two different altitudes. 60 of
them were given delayed fluid resuscitation (DFR, n = 30 at each altitude) 6 h after burn at different altitude; 60 of them were
carried out immediate fluid resuscitation (IFR, n = 30 at each altitude); 10
rats were subjected to 37°C warm water as sham burn (SG, n = 10). The Peyer’s
patches were harvested from the ileum of rats at different time point after
burn respectively. The expression of HIF-1 alpha, CD3(+) and the apoptosis and
number of T lymphocyte in Peyer’s patches were detected by tissue microarray
technology and immunohistochemistry. Results: The apoptosis was higher in DFR
group than that in IFR group. The increase in HIF-1 alpha expression was
observed mainly on cell nucleus in T lymphocytes. The expression levels of
HIF-1 alpha in Peyer’s patches were much higher in DFR group and IFR group than
those in SG, and they were higher at high altitude (3848 metres) than those at
lower altitude (1517 metres), and also higher in DFR group compared with IFR
group (all P + in Peyer’s patches were much lower in DFR
group and IFR group than those in sham group, and the lowest value appeared at
12 hours after burn (all P