TITLE:
Comparative Study of Bovine Pericardium and Gore-Tex in Tissual Interaction with Wistar Rats Diaphragm
AUTHORS:
Artur Fracassi Guerra, Mauro Razuk Filho, Neil Ferreira Novo, Willy Marcus França
KEYWORDS:
Diaphragm Hernia, Bovine Pericardium, Gore-Tex, Tissue Interaction and Wistar Rat
JOURNAL NAME:
Surgical Science,
Vol.7 No.9,
September
13,
2016
ABSTRACT: Introduction: Simple suture isn’t always possible in large
congenital diaphragmatic hernia (cdh) defects. Synthetic materials are used for
correction such as Silastic®, Gore-Tex® (GT), Teflon® or biological, such as autologous muscle patches. It was shown that bovine pericardium (bp) was
effective to correct those large defects with many positive outcomes when
compared with syntactic materials. Aim: This study aims to establish
an experimental model of correction for large diaphragmatic defect with PB and GT
patches to compare histologically the tissue interaction between them and
diaphragm in young Wistar rats. Materials & Methods: 15 wistar rats were divided in 3 groups: Rats that
used BP was named G1; Rats that used GT was named G2; and rats with only
scraping in the diphragm, named G3 (control). Animals were submited to a
laparotomy and fixed pathces to diaphragms and harvested 3 weeks later. Area between
normal diaphragm and patches were isolated and separated for histological analysis,
such as lymphocytic infiltration (inflammation), neovascularization and fibrosis. Results: G1
presented inflammation between BP and Diaphragm In 5 Samples. G2 Presented Neovascularization In 5
Samples, But No inflammation.
Fibrotic tissue overlapping GT patches occurred in 3 samples in G2. Comparing G1
with G2 there was a significant statistical difference concerning inflammation (P = 0.0079), in G1. Comparing neovascularization
there is no significant statistical difference (P = 0.4444), despite a slight higher incidence in G2. Fibrosis in both groups
presented no significant statistical difference (P = 0.4444), despite a slight higher incidence in G2. There were no
alterations in G3. Discussion: Despite the statistical difference in the inflammatory process
was more frequent in G1 (P = 0.0079),
neovascularization and fibrosis were more frequent in G2. Conclusion: The proposed
experimental model was satisfactory to reproduce suture of patches in the
diaphragm. These results suggests that inflammation, neovascularization and fibrosis
indeed contribute to a benign healing process that reacts differently in each group but can drive to a more
lasting and permanent results when biological patch is considered.
Statistical report suggests that this study should be continued with a larger sample
of animals and a wider period of time before harvest.