Article citationsMore>>
Rocha, E.S., Oliveira, J.G., Santos, J.R., Rodrigues, G.O., Figueiredo, L.B., Pessanha, J.E., Proietti, F.A., Fonseca, F.G., Bonjardim, C.A., Ferreira, P.C. and Kroon, E.G. (2013) Recombinant Envelope Protein-Based Enzyme Immunoassay for IgG Antibodies Is Comparable to Neutralization Tests for Epidemiological Studies of Dengue Infection. Journal of Virological Methods, 187, 114-120.
http://dx.doi.org/10.1016/j.jviromet.2012.09.012
has been cited by the following article:
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TITLE:
Monoclonal Antibody-Based Enzyme Immunoassay for Epidemiological Studies of Asymptomatic Dengue Infection
AUTHORS:
Supawat Chatchen, Kriengsak Limkittikul, Pimolpachr Sriburin, Arunee Sabchareon, Chukiat Sirivichayakul
KEYWORDS:
PRNT, Indirect ELISA, Asymptomatic, Dengue Infection, Ratchaburi Province Thailand
JOURNAL NAME:
Advances in Infectious Diseases,
Vol.6 No.3,
September
13,
2016
ABSTRACT: Dengue virus (DENV) is the
world most prevalent mosquito-transmitted virus. The incidence of dengue
infection has been increasing and most of the infected people are asymptomatic
or have non specific febrile illness. Laboratory test is essential to confirm
dengue infection in epidemiological studies. We developed an indirect ELISA
test based on monoclonal immunoglobulin against all four DENV serotypes and
evaluated the test for the diagnosis of asymptomatic dengue infection in paired
annual serum samples. The indirect ELISA was found to have a sensitivity 87.5%
and specificity 78.3% at optimized cut off. The results from indirect ELISA
demonstrate an incidence of asymptomatic dengue infection from children aged 4
- 11 years in Ratchaburi province, Thailand (2006-2009). These findings
indicate that the indirect ELISA is suitable for serodiagnosis and
seroepidemiological studies of asymptomatic dengue infection.
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