TITLE:
Further Pharmacological Evidence Supporting the Development of an Antiulcerogenic Drug Based on Rhizophora mangle L. Aqueous Extract. HPLC Method Proposed for Determinating a Chemical Marker
AUTHORS:
Sánchez Perera Luz María, Janet Piloto, Deyanira Canelsota, Liliam Pelzer, Betty Mancebo
KEYWORDS:
Rhizophora mangle L., Polyphenols, Intestinal Absorption, Genotoxicology, HPLC Analysis
JOURNAL NAME:
Open Access Library Journal,
Vol.3 No.1,
January
11,
2016
ABSTRACT:
Rhizophora mangle L. is a vegetal
species with high disponibility in Cuba for guarantying
economical factibility by drug development.
Preview studies showed promissory pharmacological results by the use of this
plant in the treatment of gastroduodenal ulcers. The present work involved another
evaluation of the active extract important by the development of a new drug. It
was evaluated by intestinal absorption in vitro and in situ models
and anti-diarrheic activity in rats. The polyphenolic fractions were separated
from the aqueous extract and its cytoprotective activity was studied on gastric
ulceration induced by ethanol plus hydrochloric acid in rats. Some toxicological
studies were carried out. We made a validation of HPLC method by the analysis
of one mark in the tablet development. The aqueous extract showed high
absorption, and high anti-diarrheic effect. The low-molecular weight
polyphenolic fraction showed the highest level of gastric protection. No
toxicological signs were obtained by it. HPLC method evidenced linearity, precision, sensitivity and accuracy.