TITLE:
Genetic Diversity of Tunisian and Chinese Alfalfa (Medicago sativa L.) Revealed by RAPD and ISSR Markers
AUTHORS:
Leila Touil, Aike Bao, Suomin Wang, Ali Ferchichi
KEYWORDS:
Medicago sativa L., Genetic Variability, RAPD, ISSR, UPGMA Clustering
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.6,
April
29,
2016
ABSTRACT: Medicago
sativa L.) is one of the most important forage crops in the world. The
genetic variability analysis of 19 alfalfa populations collected from three
sites in South Tunisia (Gabes, Kebili, Tozeur) and 1 from North West China were carried out using Random Amplified
Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) markers. Five
RAPD primers amplified 44 bands of which 22 were polymorphic; and five ISSR
primers amplified 51 bands of which 33 were polymorphic. The percentage of
polymorphic bands detected by RAPD and ISSR was 50% and 64.7%, respectively.
The resolving power (Rp) varied between 0.6 and 4.1 with an average of 2.02 for
RAPD marker and between 0.7 and 6.5 with an average of 2.28 for ISSR marker.
However the Average Informativeness band (AvIb) was ranged from 0.2 to 0.9 with
an average of 0.5 in RAPD marker and from 0.29 to 0.7 with an average of 0.624
in ISSR marker. The RAPD marker revealed less within population genetic
diversity than ISSR marker. Although Cluster (UPGMA) and Correspondence
Factorial Analyses (CFA) indicate that populations’ clustering made independently
both from the geographical origin.